中国免疫学杂志2011，Vol.27Issue(3)：228-231,4.DOI:10.3969/j.issn.1000-484X.2011.03.008
茯苓多糖对免疫抑制小鼠粘膜淋巴组织及脾脏中CD3+和CD19+细胞变化的影响
The effect of Pachymaran on the change in lymphocyte subsets induced by cyclophosphamide
摘要
Abstract
Objective :To study the effect of Pachymaran on the change in lymphocyte subsets induced by cyclophosphamide ,and to explore the different elfects of Pachymaran on the intestinal mucosal immunity and other peripheral immunity .Methods :The immunosuppressive mice were produced by intraperitoneal injection of cyclophosphamide .Lymphocytes were isolated from PPs ,MLNs and SP .Then the proportion of CD3+ and CD19+ cells was detected with flow cytometry .Results :The proportion of CD3+ and CD19+ cells was changed significantly in immunosuppressive mice .The proportion of CD3+ cells was increased ,while CD19+ cells were decreased .Pachymaran could resume the proportion of CD3+ and CD19+ cells in PPs and MLNs of the immunosuppressive mice ,but did not the proportion of CD3+ and CD19+ cells in SP .Conclusion : Pachymaran can antagoruze the changes induced by cyclophosphamide ,suggesting that the effect of oral administration of Pachymaran on intestinal mucosal immunity system is more obviously than on the other parts of immunitv system .关键词
茯苓多糖/环磷酰胺/淋巴细胞/小鼠分类
医药卫生引用本文复制引用
王青,胡明华,董燕,潘华新,周联,王培训..茯苓多糖对免疫抑制小鼠粘膜淋巴组织及脾脏中CD3+和CD19+细胞变化的影响[J].中国免疫学杂志,2011,27(3):228-231,4.基金项目
本文受国家自然科学基金项目(30873415)和教育部博士点科研基金项目(200805720001)资助 （30873415）
