中国免疫学杂志2011，Vol.27Issue(3)：200-203,4.DOI:10.3969/j.issn.1000-484X.2011.03.002
人CK8&18阳性胸腺上皮细胞对脐血CD34+细胞向T细胞分化的影响
The effects of the human CK8/18 positive thymic epithelial cells on differentiation of cord blood CD34+ cells to T cells
摘要
Abstract
Objective :To study the effects of CK8/18 positive thymic epithlial cell (TEC ) on T cells proliferation and differentiation , we established a model by co-cultured CK8/18 positive TEC and cord blood mononuclear cells (CBMC ) in transwell co-culture system .Methods :Thymic epithelial cells were obtained through collagenase digestion culture and identified with TEC keratin (CK ) and CK8/18 antigen . CD34+ cells were collected and purified from CBMC by means of imunomagnetic beads on MiniMACS device .The original culture of CK8/18 positive TEC was put into transwell upper chamber (pore size 5 μm) ,and then the CD34+ cells were added to transwell upper chamber until the PC membrane were completely covered by TEC ,when the cells into the lower chamber after 48 hours were detected with flow cytometry .Results :The TEC were anti-CK-positive and anti-CK8/18 staining positive .Compared with the control ,the CD3+ CD4- CD8 double negative cells ,CD3+ CD4 + CD8- single and CD45RO+ cells that entered the transwell lower chamber increased significantly ( P＜0 .01 ) in transwell co-culture system .Conclusion :CK8/18 positive thymic epithelial cells can promote CBMC CD4+ single-positive T cells and CD45RO+ cells proliferation .关键词
胸腺上皮细胞/脐血单个核细胞/T细胞亚群分类
医药卫生引用本文复制引用
李倩如,杜焱,苏艳宾,杜英,张清勇,李国喜,胡祥..人CK8&18阳性胸腺上皮细胞对脐血CD34+细胞向T细胞分化的影响[J].中国免疫学杂志,2011,27(3):200-203,4.基金项目
本文受国家自然科学基金项目(No.30570625)、河南省自然科学基金(No.511042700)及广东省自然科学基金(No.2007B031500015)资助 （No.30570625）
