中国人兽共患病学报2011，Vol.27Issue(3)：241-245,5.
布鲁氏菌PCR鉴定方法的研究与应用
Study and application of polymerase chain reaction assay on Brucella identification
摘要
Abstract
The purpose of this study was to apply PCR and PCR-SSCP assay method to identify Brucella and specific type of Brucella. In order to analyze a great number of partition Brucella of types sequence which have been issued as specific genotype and search for the distributing role of genotype-specific nucleotides, the specific primers were designed and PCR method was applied to identify specific type of Brucella. PCR-SSCP method was established to distinguish vaccine strain Al9 from other specific types of Brucella. Results indicated that the established PCR method could be used to conduct the diagnosis of Brucellosis and identify specific type of Brucella. And PCR-SSCP assay could detect vaccine strain Al9, It's suggested that PCR and PCR-SSCP assay is a reliable and convenient method to identify specific type of Brucella and vaccine strain Al9.关键词
布鲁氏菌/鉴定/PCR/PCR-SSCP分类
医药卫生引用本文复制引用
钟旗,易新萍,李博,吴冬玲,刘丽娅,叶锋,闫晶华,李金平,王力俭..布鲁氏菌PCR鉴定方法的研究与应用[J].中国人兽共患病学报,2011,27(3):241-245,5.基金项目
农业公益性行业科研专项经费(2008030261) （2008030261）
国家高技术研究发展计划(2007AA02Z405) （2007AA02Z405）
新疆维吾尔自治区高技术研究发展计划(200911109) （200911109）
