中国病理生理杂志2011，Vol.27Issue(3)：481-487,7.DOI:10.3969/j.issn.1000-4718.2011.03.012
白花丹醌增强TRAIL诱导Kasumi-1细胞凋亡及其机制
Plumbagin enhances TRAIL-induced apoptosis in Kasumi-1 cells
摘要
Abstract
AIM : To investigate the effect of plumbagin and tumor necrosis factor - related apoptosis - inducing ligand ( TRAIL ) on the apoptosis of leukemic Kasumi - 1 cells.METHODS: Kasumi - 1 cells were treated with plumbagin alone, recombinant soluble TRAIL( rsTRAIL ) alone or the combination of plumbagin with rsTRAIL to induce apoptosis.The cell proliferation was analyzed by CCK - 8 assay.Apoptosis was determined by flow cytometry with Annexin V/PI double staining and TUNEL staining.The expression of DR4 and DR5 at mRNA level was measured by real - time PCR.The expression of signal transduction proteins, such as DR5 , caspase - 3, caspase - 8, caspasep -9, Bid, Bax and c - FLIP was detected by Western blotting.RESULTS : Both rsTRAIL and plumbagin induced the apoptosis in Kasumi cells, and combination of plumbagin with rsTRAIL enhanced the apoptosis.The ratios of Annexin V - positive Kasumi 1 cells were ( 27.7 ±2.9 )％ , ( 25.6 ±3.1 )％ and ( 52.1 ±3.3 )％ in 100 μg/L rsTRAIL group, 2 μ mol/L plumbagin group and the combination group, respectively, and the positive rate in comhination group was significantly higher than those in other 2 groups.TUNEL assay demonstrated that the number of apoptotic cells in combination group was higher than that in the cells treated with rsTRAIL or plumbagin alone.Plumbagin up - regulated the expression of DR5 at mRNA level in Kasumi - 1 cells, and up - regulation of DR5 , activation of caspase - 8 and down - regulation of c - FLIP at protein level were detected in the cells treated with plumbagin alone and the comhination of plumhagin with rsTRAIL.CONCLUSION : Plumbagin enhances TRAIL - induced apoptosis in Kasumi - 1 cells by up - regulating DR5 , activating caspase - 8and down - regulating c - FLIP.关键词
Kasumi-1细胞/细胞凋亡/白花丹醌/肿瘤坏死因子相关凋亡诱导配体/基因,DR5/基因,DR4分类
医药卫生引用本文复制引用
徐同鹏,刘立根,蒋雪玮,高武,谢英华,赵莉敏,韩曦瑶,陆道培..白花丹醌增强TRAIL诱导Kasumi-1细胞凋亡及其机制[J].中国病理生理杂志,2011,27(3):481-487,7.基金项目
国家自然科学基金资助项目(No.30672415) （No.30672415）
上海市科委攻关项目(No.054119528) （No.054119528）
