中国病理生理杂志2011，Vol.27Issue(3)：539-544,6.DOI:10.3969/j.issn.1000-4718.2011.03.023
新型大麻制剂O-1602和CBD减轻小鼠实验性急性胰腺炎的机制研究
Effects of new kinds of cannabis preparations O-1602 and cannabidiol on experimental acute pancreatitis
摘要
Abstract
AIM : To investigate the therapeutic effects of O - 1602 and cannabidiol ( CBD ), the new kinds of cannahis preparations, on caerulein ( CAE ) - induced acute pancreatitis ( AP ) in mice.METHODS : AP was induced by intraperitoneal injection ( ip ) of CAE in mice ( 50 μg/kg hourly with a total of 6 times), and the mice in control group were given normal saline ( NS ) ip in stead of CAE in the same way.The AP mice were administrated O - 1602 or CBD for the therapeutic evaluation by observing the following parameters : pathological changes of pancreatic tissue, plasma activity of amylase and lipase ( biochemical methods ) , the levels of TNF - α and IL - 6 in the plasma ( ELISA ) , and the activity of myeloperoxidase ( MPO ) in the lung ( biochemical methods ).Meanwhile, real - time PCR and Western blotting were used to evaluate the expression of heat shock protein 60 ( HSP60 ) at mRNA and protein levels, respectively.RESULTS : The pancreatic tissues in AP group appeared obvious edema and neutrophil infiltration, which were significantly improved by treating with AP + O - 1602 or AP + CBD.The activity of amylase , lipase and MPO, as well as the levels of TNF - α and IL - 6 in AP group significantly increased compared with NS group ( P ＜ 0.05 ), while these parameters were significantly lower in AP + O - 1602 group and AP + CBD group than those in AP group.Meanwhile, the expression of HSP60 at mRNA and protein levels in pancreas tissues was reduced in AP group ( P ＜ 0.05 ), and was improved to some extent after treating with O - 1602 or CBD ( P ＜0.05 ).CONCLUSION : The O - 1602 and CBD show anti - inflammatory effects on CAE induced AP in mice and the mechanisms might be related to the effects of cannabinoids on inhibiting inflammatory mediators and cytokines, and increasing the expression of cytoprotective factor HSP60.关键词
急性胰腺炎/大麻素类/热休克蛋白质60分类
医药卫生引用本文复制引用
冯佳燕,李琨,林旭红,余良英,李永渝..新型大麻制剂O-1602和CBD减轻小鼠实验性急性胰腺炎的机制研究[J].中国病理生理杂志,2011,27(3):539-544,6.基金项目
国家自然科学基金资助项目(No.30971168) （No.30971168）
