中国病理生理杂志2011，Vol.27Issue(3)：460-463,4.DOI:10.3969/j.issn.1000-4718.2011.03.008
辛伐他汀对尼古丁诱导脐静脉内皮细胞分泌t-PA和PAI-1的影响
Effects of simvastatin on nicotine-induced t-PA and PAI-1 expression in human umbilical vein endothelial cells
摘要
Abstract
AIM : To investigate the effects of simvastatin on nicotine - induced expression of tissue plasminogen activator ( t - PA ) and plasminogen activator inhibitor - 1 ( PAI - 1 ) at mRNA and protein levels in human umbilical vein endothelial cells ( HUVECs ).METHODS : Cultured HUVECs at passage 3 to 6 were randomly divided into control group, nicotine group and simvastatin groups.The cells in simvastatin groups were treated with simvastatin at the concentrations of 1, 10 and 100 μmol/L for 2 h, and then exposed to nicotine at the concentration of 100 μmol/L for 24 h.The production of t - PA and PAI - 1 in the cultured medium was measured by enzyme - linked immunosorbent assay ( EIISA ).The mRNA expression of t - PA and PAI - 1 was determined by reverse transcript - polymerase chain reaction ( RT - PCR ).RESULTS: Compared with the control cells, the secretion and mRNA expression of PAI - 1 in the cells treated with nicotine were significantly increased( P ＜ 0.05 ).Simvastatin decreased the secretion and mRNA expression of PAI - 1 in HUVECs stimulated by nicotine in a dose - dependent manner, and the most notable inhibitory effect was observed in 100μmol/L simvastatin group, in which the level of PAI - 1 was almost the same as that in the control cells ( P ＞0.05 ).Compared with the control cells , nicotine inhibited the mRNA expression of t - PA in HUVECs ( P ＜ 0.05 ), and simvastatin at the concentrations of 10 and 100 μmol/L markedly increased the mRNA expression of t - PA in HUVECs treated with nicotine.However, the production of t - PA remained no significantly different among groups ( P ＞ 0.05 ).CONCLUSION: Simvastatin ohviously decreases the expression of PAI - 1 at mRNA and protein levels, increases the mRNA expression of t - PA, and improves the fibrinolytic function of HUVECs exposed to nicotine in vitro.关键词
辛伐他汀/尼古丁/人脐静脉内皮细胞/组织纤溶酶原激活物/纤溶酶原激活物抑制物1分类
医药卫生引用本文复制引用
扈丽娟,胡晓芸,杜艳,韩葆芬..辛伐他汀对尼古丁诱导脐静脉内皮细胞分泌t-PA和PAI-1的影响[J].中国病理生理杂志,2011,27(3):460-463,4.基金项目
太原市科技兴市资助项目(No.0904051) （No.0904051）
