植物学报2011，Vol.46Issue(2)：147-154,8.DOI:10.3724/SP.J.1259.2011.00147
拟南芥GLP13基因在植物抗氧化胁迫响应中的作用
Function of GLP13 in Response to Plant Oxidative Stress in Arabidopsis
摘要
Abstract
Germin-like proteins (GLPs) comprise a large family of soluble extracellular matrix glycoprotein, which is similar to wheat germin and plays an important role in growth and development and in biotic and abiotic stress responses of plants. To understand the role of GLP13 in planta, we investigated its expression patterns; isolated and characterized a knock-down mutation in the GLP13 gene (named glp13); and constructed overexpression GLP13 plants (named 35S::GLP13). Compared to the wild type, the glp13 mutant showed a lower cotyledon green ratio and more restrained root growth when 3 different types of plants were treated with methyl viologen (MV); however, the cotyledon green ratio was higher and the root growth less restrained in 35S::GLP13 plants. The chlorophyll fluorescence parameter Fv/Fm of 35S::GLP13 plants decreased slower than in the wild type after 2-week MV treatment of seedlings. The expression of oxidative stress response genes (CSD1, FSD1, UGT71C1 and CAT1) were analyzed by semi-quantitative RT-PCR; the expression of FSD1 was increased in 35S::GLP13 plants, with no differences in the expression level of CAT1, CSD1 and UGT71C1 among 3 different types of plants after 4-hr treatment with MV. Our results indicate that GLP13 plays an important role in response to oxidative stress in Arabidopsis.关键词
拟南芥/类萌发素蛋白/甲基紫精/氧化胁迫引用本文复制引用
唐源江,闵伶俐,高桂兰,杜金菊,杨浪,阳成伟..拟南芥GLP13基因在植物抗氧化胁迫响应中的作用[J].植物学报,2011,46(2):147-154,8.基金项目
国家自然科学基金(No.30770201)和广东省科技攻关项目(No.2007B020701005) （No.30770201）
