中国人兽共患病学报2011，Vol.27Issue(3)：190-195,6.
重组蛋白ASP-1对高致病性禽流感M2e疫苗分子的佐剂活性
Adjuvanticity of recombinant protein ASP-1 on M2e-based vaccine against highly pathogenic avian influenza vaccine
摘要
Abstract
In order to study the adjuvanticity of recombinant protein ASP-1 on M2e-based vaccine against highly pathogenic avian influenza, the recombinant protein TrxA-M2e3 serving as M2e-based vaccine against highly pathogenic avian influenza was expressed in prokaryotic system, and the vector protein trxA of pET32a(+) was fused to M2e3. The truncated segment ASP-1 of Onchocerca volvulus activation associated protein was expressed in E. coli as adjuvant protein. And the mice were vaccinated with purified TrxA-M2e3 and ASP-1 proteins combinedly or separately. M2e-specific IgG antibody in mouse sera were measured. Then vaccinated mice were challenged with lethal dose of different clades of H5N1 viruses to detect the cross-protection. Results displayed that the purified recombinant proteins, TrxA-M2e3 and ASP-1 were generated. Comparing with the group vaccinated with TrxA-M2e3 alone, the titers of M2e-specific antibody in mouse sera were significantly enhanced in the presence of adjuvant protein ASP-1. After lethal challenges of different clades of H5N1 viruses, the viral replications in lungs of mice vaccinated with TrxA-M2e3 plus ASP-1 were significantly inhibited, and all mice in this group survived, while all mice in other groups died. It's suggested that M2e is the ideal antigen target for developing the novel avian influenza vaccine to confront the viral variations. And the recombinant protein ASP-1 is a promising novel adjuvant which playing a key role on M2e-inducing cross-protections.关键词
高致病性禽流感/M2e/ASP-1/疫苗/佐剂分类
医药卫生引用本文复制引用
俞建萍,周育森,赵光宇,肖文珺,郭彦,孙世惠,寇志华,于虹,温晶,何后军..重组蛋白ASP-1对高致病性禽流感M2e疫苗分子的佐剂活性[J].中国人兽共患病学报,2011,27(3):190-195,6.基金项目
国家自然科学基金(30901371)和(31000412)资助 （30901371）
