中国人兽共患病学报2011，Vol.27Issue(3)：176-179,4.
检测肠道病毒71型RT-LAMP技术的建立及其应用
Establishment and application of RT-LAMP technique in detection of enterovirus 71 gene
摘要
Abstract
A simple and sensitive Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) method was established to provide a new rapid method for detecting Enterovirus 71 gene. A set of six specially primers that recognized eight distinct sequences of VP1 gene of EV71 were designed, RT-LAMP method was established, and the specificity and sensitivity of RT-LAMP were verified. The stool or throat specimens of patients infected with Hand Foot and Mouth Disease (HFMD) were tested by RT-LAMP technique, and the result was compared to that of RT-PCR and Real-time PCR. Results demonstrated that the RT-LAMP technique has been successfully detected VP1 gene of EV71 , and showed a good consistency with Real-time PCR results but higher detection rates than RT-PCR method. It's suggested that RT-LAMP technique appeared to be high sensitivity and specificity, as well as simple and rapid. It is suitable for the diagnosis of HFMD and for molecular epidemiological surveillance of infection, and with good development prospects.关键词
肠道病毒71型/手足口病/逆转录环介导等温核酸扩增技术分类
医药卫生引用本文复制引用
耿英芝,姚文清,郭军巧,于伟,毛玲玲,陈静乙,安春丽..检测肠道病毒71型RT-LAMP技术的建立及其应用[J].中国人兽共患病学报,2011,27(3):176-179,4.基金项目
国家自然科学基金(30670916) （30670916）
