作物研究2011，Vol.25Issue(1)：56-60,5.DOI:10.3969/j.issn.1001-5280.2011.01.17
大叶冬青苦丁茶多糖提取、纯化与抗氧化活性研究
Extraction,Purification and Antioxidant Activity in Vitro of Polysaccharides from Kudingcha Made from Ilex latifolia Thunb
摘要
Abstract
Kudingcha made from Ilex latifolia Thunb was defatted with 85％ ethanol, and then extracted with hot water,decolored by S-8 macroporous resin, precipitated by ethanol, and dried to afford crude polysaccharide (ILPS). Four purified polysaccharides, ILPS-1, ILPS-2, ILPS-3 and ILPS-4, were gained from crude ILPS by DEAE-52 anion-exchange chromatography. Furthermore. the antioxidant activities in vitro of the crude ILPS and its purified fractions were evaluated by determinations of scavenging activities on 1. 1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, superoxide anion radicals (O2· - ), hydroxyl radicals (·OH) , ferric reducing-antioxidant power (FRAP) and chelating capacity to Fe2+. The results indicated that ILPS exhibited strong antioxidant activity, and the antioxidant activity was significantly correlated with its concentration.关键词
大叶冬青苦丁茶/多糖/提取/纯化/抗氧化活性分类
医药卫生引用本文复制引用
赵天湖,范嘉龙,闫冬,徐人杰,郎昌野,孙怡,曾晓雄..大叶冬青苦丁茶多糖提取、纯化与抗氧化活性研究[J].作物研究,2011,25(1):56-60,5.基金项目
国家大学生创新性实验计划项目(091030727). （091030727）
