作物学报2011，Vol.37Issue(3)：405-414,10.DOI:10.3724/SP.J.1006.2011.00405
杂交粳稻亲本米质性状优异配合力的标记基因型鉴定
Identification of Marker Genotypes Associated with Elite Combining Ability for Quality Traits in Parents of Hybrid Japonica Rice(Oryza sativa L.)
摘要
Abstract
The area planting japonica hybrid rice is only 3％ of the total area (8.28×106 ha) of japonica rice. One of the reasons why japonica hybrid rice popularized slowly is that grain quality of hybrid was inferior to that of conventional cultivars. In order to improve grain quality combining ability of parental lines more efficiently, SSR marker genotypes of elite combining ability for 10 quality traits, namely, brown rice rate (BRR), milled rice rate (MRR), head rice rate (HRR), grain length (GL), grain width (GW), percentage of chalky grain (PCG), chalkiness degree (CD), gelatinization temperature (GT), gel consistency (GC), and amylose contents (AC), were identified in this study, by analyzing the data of combining ability and SSR markers in six CMS lines and twelve restorer lines, which were genotyped using 115 pairs of SSR primers. A total of 30 SSR marker genotypes were significantly associated with combining ability of the 10 quality traits. Twenty-five of them were marker genotypes for unfavorable combining ability of quality traits. The remaining five marker genotypes were associated with elite combining ability for quality traits of parents. RM263-175/180 and RM444-230/240 were marker genotypes of elite combining ability for HRR, which increased 3.2％ and 2.5％ of HRR in F1, respectively. RM3-120/150 shortened GL value in F1 by 2.4％. RM444-180/240 increased GW trait value of Ft by 2.1％. RM428-273/294 decreased AC trait value of hybrid rice harvested from F1 plants by 7.0％. Among the SSR marker genotypes associated with combining ability of the 10 quality traits, eight marker genotypes also affected combining ability of yield components simultaneously. RM3-120/150 increased simultaneously total spikelets per plant (TSP) and filled spikelets per plant (FSP) in F1 by 15.9％ and 10.9％, respectively. RM1211-150/160 decreased trait values of BRR and MRR in F1 by 0.9％ and 1.1％, respectively, and simultaneously increased trait values of TSP and FSP in F1 by 21.8％ and 20.4％.RM23-150/160 increased trait values of PCG and CD in F1 by 44.1％ and 45.7％, and simultaneously increased daily yield per plant and TSP in F1 by 11.2％ and 11.6％, respectively. These results could be used for directing improvement of combining ability of parents for quality traits and yield traits through the marker-assisted selection, and for determining parents for making combinations of hybrid japonica rice in the future.关键词
粳稻/配合力/SSR标记/米质/标记基因型引用本文复制引用
黄殿成,马文霞,洪德林,梁奎,孙程,江建华,石明亮,戴剑,谢辉,赵凯铭,阮方松..杂交粳稻亲本米质性状优异配合力的标记基因型鉴定[J].作物学报,2011,37(3):405-414,10.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2010AA101301),高等学校学科创新引智计划项目(B08025),引进国际先进农业科学技术计划(948计划)项目(2006-G8[4]-31-1)和教育部科技基础条件平台重点资助项目(505005)资助. （863计划）
