作物学报2011，Vol.37Issue(3)：469-476,8.DOI:10.3724/SP.J.1006.2011.00469
基于单片段代换系的水稻粒型QTL加性及上位性效应分析
Analysis of Epistatic and Additive Effects of QTLs for Grain Shape Using Single Segment Substitution Lines in Rice(Oryza sativa L.)
摘要
Abstract
It is important to accelerate breeding process by pyramiding genes purposefully with molecular marker-assisted selection methods. Additive and epistatic effects of genes are the key to the success in genes pyramiding breeding. In this paper, additive and epistatic effects of QTLs for rice grain shape were analyzed using 16 single segment substitution lines (SSSL) and 15 double segment substitution lines (DSSL). A total of nine QTLs were identified on the chromosomes 2, 3, 4, 7, and 10 respectively, containing four for grain length, one for grain width and four for ratio of grain length to width. Furthermore, seven pairs of digenic interactions were detected for the grain shape. Among them, three interactions occurred between two loci with obvious effects on the traits, one interaction did not have obvious effects at both two loci, and three interactions occurred between one locus with and another without main effect at the single-locus level. Again, the results also indicated that the epistatic effects were different when the same QTL controlling grain length was pyramided with various SSSLs. Only when the epistatic effects were syntropic with the additive effects of the target genes, the pyramiding effect could be obvious. Moreover, longer or shorter grain was difficult to be obtained by pyramiding two QTLs controlling long grain or two QTLs for short grain. The results are important for improving grain shape by molecular marker assisted selection.关键词
水稻/单片段代换系/粒型QTL/加性效应/上位性效应引用本文复制引用
赵芳明,张桂权,曾瑞珍,杨正林,凌英华,桑贤春,何光华..基于单片段代换系的水稻粒型QTL加性及上位性效应分析[J].作物学报,2011,37(3):469-476,8.基金项目
由国家自然科学基金重点项目(30330370),重庆市自然科学基金项目(CSTC,2006BB1330),西南大学博士点基金项目和西南大学博士后基金项目资助. （30330370）
