中国病理生理杂志2011，Vol.27Issue(3)：488-493,6.DOI:10.3969/j.issn.1000-4718.2011.03.013
量子点免疫荧光技术检测EMMPRIN、MMP-2和P53蛋白在人肺癌组织中的表达意义
Protein expression of EMMPRIN,MMP-2 and P53 detected by technique of quantum dots immunofluorescence in human lung cancer
摘要
Abstract
AIM : To investigate the expression of extracellular matrix metalloproteinase inducer ( EMMPRIN/CD147 ), matrix metalloproteinase2 ( MMP - 2 ) and P53 proteins in human lung cancer tissues and to explore the relationship between EMMPRIN protein and malignant biological behaviour of lung cancer.METHODS : Fluorescent semiconductor nanocrystals [ also known as quantum dots ( QDs ) ] were applied, which were nanometer - sized light - emitting particles and were emerging as a new class of fluorescent probes for cancer detection due to the unique optical and electronic properties.The technique of QDs immunofluorescence histochemistry ( QDs - IHC ) was used to detect the protein expression of EMMPRIN.P53 and MMP -2 in the human lung cancer microarray, and co - expression of EMMPRIN/P53 proteins was also simultaneously detected by douhle - laheling immunofluorescence.RESULTS : Compared with non - cancer ous lung tissues, the positive rates of EMMPRIN, P53 and MMP -2 proteins in the lung cancer tissues were 70.00％ ,77.14％ and 72.86％ , respectively, and the differences were all significant ( P ＜ 0.05 ).The positive rates of EMMPRIN ,P53 and MMP -2 proteins were all significantly related with tumor staging( TNM stage ) and lymph node metastasis ( P ＜0.05 ).The positive correlation between EMMPRIN expression and protein levels of MMP - 2 and P53 was observed ( P ＜0.01 ).CONCLUSION : The protein expression of EMMPRIN, P53 and MMP -2 is correlated with the development of lung cancer.Malignant progression of lung cancer promoted by EMMPRIN may be closely related with the expression of MMP - 2and P53.关键词
肺肿瘤/细胞外基质金属蛋白酶诱导因子/基质金属蛋白酶2/量子点分类
医药卫生引用本文复制引用
潘琦,陈福春,武百强,鲍军,薛洁皓,陈洪雷..量子点免疫荧光技术检测EMMPRIN、MMP-2和P53蛋白在人肺癌组织中的表达意义[J].中国病理生理杂志,2011,27(3):488-493,6.基金项目
国家自然科学基金资助项目(No.30900652) （No.30900652）
浙江省中医药普通课题研究计划资助项目(No.2008CB077) （No.2008CB077）
