中国药房2011，Vol.22Issue(11)：1007-1009,3.
钩藤HPLC指纹图谱的构建
Establishment of HPLC Fingerprint of Ramulus Uncariae Cum Uncis
摘要
Abstract
OBJECTIVE: To establish the HPLC fingerprint of Ramulus Uncariae Cum Uncis, and to provide objective basis for the quality control of Ramulus Uncariae Cum Uncis based on the results of the content determination of rhynchophylline and isorhynchophylline. METHODS: The determination was performed on Hypersil BDS C18(250 mm×4.6 mm, 5 μm) column with the mobile phase consisted of methanol-0.02％ triethylamine-water (gradient elution). The flow rate was 1.0 mL· min-1 and UV detection wavelength was 254 nm. RESULTS: The HPLC fingerprints of Ramulus Uncariae Cum Uncis were established according to the chromatographic data of 10 batches of samples. The results of similarity evaluation and principle component analysis (PCA)were favorable. CONCLUSION: Established fingerprint reflects the inherent character of Ramulus Uncariae Cum Uncis and can be used for the quality evaluation of it.关键词
钩藤/高效液相色谱法/指纹图谱分类
医药卫生引用本文复制引用
何昱,黄家卫,杨维..钩藤HPLC指纹图谱的构建[J].中国药房,2011,22(11):1007-1009,3.基金项目
浙江中医药大学科研基金重点项目(17107003) （17107003）
