中国药房2011，Vol.22Issue(11)：974-976,3.
LC-MS/MS法测定大鼠肝微粒体孵育液中靛玉红的含量
Determination of Indirubin in Rat Hepatomicrosome Incubation Solution Using LC-MS/MS
杨波 1冉健莉 1刘松青1
作者信息
- 1. 第三军医大学第一附属医院药剂科,重庆市,400038
- 折叠
摘要
Abstract
OBJECTIVE: To establish LC-MS/MS method for the content determination of indirubin in rat hepatomicrosome incubation solution.METHODS: A liquid-liquid extraction procedure was used for the processing of hepatomicrosome incubation solution through the LC-MS/MS method The extracts were separated by HPLC using methanol-10 mmol· L-1 ammonium acetate (pH value adjusted to 7.40) (90∶ 10) as mobile phase.Selected reaction monitoring (SRM) mode with the transition of m/z 262.9→219.0 (indirubin) and m/z 531.0→82.1 (ketoconazole, IS) was used for quantitative analysis.RESULTS: The determination of indirubin was completed within 5 min.The linear range of indirubin was 1-100 ng·mL-1 with average recovery of 94.0％-104.4％.The RSD of intra-day and inter-day were lower than 3.6％ and 13.4％, and the limit of detection was 0.5 ng·mL-1.CONCLUSION: The method is accurate, sensitive and rapid for the determination of indirubin in rat hepatomicrosome incubation solution.关键词
靛玉红/液相色谱-电喷雾串联质谱法/肝微粒体孵育液/大鼠分类
医药卫生引用本文复制引用
杨波,冉健莉,刘松青..LC-MS/MS法测定大鼠肝微粒体孵育液中靛玉红的含量[J].中国药房,2011,22(11):974-976,3.
