重庆医学2011，Vol.40Issue(11)：1041-1043,3.DOI:10.3969/j.issn.1671-8348.2011.11.001
维生素D缺乏动物模型的建立
To establish the animal model of vitamin D deficiency
摘要
Abstract
Objective To establish and evaluate the animal model of vitamin d deficiency using BALB/c mice.Methods Put BALB/c mice into normal group, VD+ group andVD- group,measured the weight of the three groups of 4 -10,12th week; observed the mouse activity; Recorded food consumption during 8 and 12th week;Assay for serum albumin and 25-hydroxyvitamin D level after 12 weeks.Then 10 mouse of vitamin D deficiency were divided into two groups with normal diet for six weeks, but one group as beginning the other one as normal group,then measured the serum level of 25-hydroxyvitamin D.Results Normal group and VD+ group had no statistical differences in body weight(P＞0.05) ; At 8th week ,the average consumption of synthesis food in VD+ group was (6.72±0.89) g/d , VD- group was (5.82±1.34) g/d ; At 12th week times VD+ group was ( 7.05± 0.72) g/d ,VD- group was ( 6.670±0.84 ) g/d, there was a little difference at 8th week but no statistical differences at 12th weeks; The plasma albumin of normal group, VD+ group, VD- group were (33.2±1.04) , (32. 4±0.53) , (32.8±0.67) g/L, respectively.Two of the three groups and between the two groups were no statistically differences; Serum 25-hydroxyvitamin D had no significant differences between normal group and VD+ group,but significant differences in VD- group with decreased into normal 20％ ;The serum 25hydroxyvitamin D of two deficiency groups after six months were (18.46±1.53) and (58.46±5.53)nmol/L(P＜0.001).Conclusion The establishment of vitamin deficiency BALB/c mice animal model by controlling the sources of vitaminD is feasible.关键词
维生素D缺乏/维生素D生理作用/BALB/c小鼠/动物模型引用本文复制引用
常正奇,张泽华,罗飞,许建中..维生素D缺乏动物模型的建立[J].重庆医学,2011,40(11):1041-1043,3.基金项目
国家自然科学基金青年基金资助项目(30901174). （30901174）
