广东医学2011，Vol.32Issue(5)：582-584,3.
实时荧光PCR技术在丙型肝炎病毒基因型检测中的应用
Application of real - time fluorescent PCR in the detection of HCV genotypes.
摘要
Abstract
Objective To study the application of real - time fluorescent PCR in the detection of Hepatitis C Virus ( HCV ) genotypes. Methods Real - time fluorescent PCR with one step reverse transcription ( RT ) combining with TaqMan was performed to detect the HCV genotypes. And sequencing of NS5B gene was applied for comparison. Results Real -time fluorescent PCR genotyping was successfully performed in 110 of 119 cases of HCV, with 71 and 48 cases were genotype - 1 and non - genotype - 1, respectively. Genotype - 1 and non - genotype - 1 were revealed in 73 cases ( 66.4％, all were subtype lb ) and 37 cases ( 33.6％, 7 cases were 2a, 6 cases were 3a, 3 cases were 3b, 1 case was 4a, 1 case was 4k, 1 case was 5a, 17 cases were 6a and 1 case was 6n ), respectively. The total coincidence rates of genotype - 1 and non - genotype - 1 were 71.8％ ( 79/110 and 80. 9％ ( 55/68 ), respectively. Conclusion Real - time fluorescent PCR is rapid and simple test for HCV genotyping, though without high specificity.关键词
实时荧光PCR/基因测序/丙型肝炎病毒/基因型Key words
real -time fluorescent PCR/ sequencing/ hepatitis C virus/ genotype引用本文复制引用
袁小珍,陈伟烈,魏绍静,唐漾波..实时荧光PCR技术在丙型肝炎病毒基因型检测中的应用[J].广东医学,2011,32(5):582-584,3.基金项目
广东省医学科研基金资助项目(编号:A2009550),广州市医药卫生科技重点项目(编号:2007-ZDi-03) （编号:A2009550）
