广西医学2011，Vol.33Issue(2)：174-176,3.
165例小儿肾穿刺组织免疫荧光病理检查技术分析
Pathological Immunofluorescence Study on 165 Cases of Renal Biopsy in Children
苗小芬 1王睿 1朱雪明2
作者信息
- 1. 江苏省苏州市中医院病理科,苏州市,215009
- 2. 苏州大学附属儿童医院病理科,苏州市,215003
- 折叠
摘要
Abstract
Objective To investigate diagnostic value of the renal biopsy in children with kidney disease.Methods For ultrasound guided percutaneous renal biopsy, Both paraffin and frozen specimens were inspected.Paraffin sections were done for HE , PAS, PASM , and Masson staining, and the frozen sections were prepared for IgG ,IgM , IgA , C3 and C1q. The method of sample processing was modified to improve production quality( Cuting up, Fixing,Dyeing, etc ). Results On the paraffin sections with the four staining methods, a variety of pathological changes was observed with contrast clearly, and a sufficient number of glomeruli over ten was seen on each section. The number of glomeruli over five was observed on immunofluorescence staining of frozen section with clear background. No ice crystals formed , and the fluorescent marker was clear. Positive parts of lumps or linear or granular yellow-green fluorescence staining were seen at the glomerular lesion sites corresponding to the type of the kidney diseases. Conclusion A diagnosis may be made by analyzing the various results with significant improvement in the diagnostic rate of different kidnev diseases.关键词
肾病/免疫荧光/肾穿刺活检/小儿分类
医药卫生引用本文复制引用
苗小芬,王睿,朱雪明..165例小儿肾穿刺组织免疫荧光病理检查技术分析[J].广西医学,2011,33(2):174-176,3.
