江苏大学学报（医学版）2011，Vol.21Issue(2)：93-96,封2,5.
成人骨髓间质干细胞向神经干细胞的诱导分化
Induced differentiation of adult human BMSCs into neural stem cells
摘要
Abstract
Objective : To establish an in vitro method to incluce the differentiation of adult human bone marrow mesenchymal stem cells ( BMSCs) into neural stem cells as the seed cells for the transplant therapy of neural system injuries. Methods : Human BMSCs were isolated from bone marrow and cultured for three passages ( P3 ) . The BMSCs were plated to the agarose-coated anti-adherent flasks , using the neural stem cell culturing medium including B27 , EGF and bFGF to induce the differentiation of BMSCs to neurospheres. With the passage of neurospheres , neural stem cells ( NSCs) proliferated. At last the NSCs markers in adherent cultured BMSCs and anti-adherent plus inducing cultured cells grew as neurospheres were compared with immunofluorescence and Western blotting . Results : Anti-adherent plus inducing culture can derive neurospheres from BMSCs . The primary neurospheres can be passaged to secondary neurospheres with proliferation. The cells in neurospheres expressed sonic hedgehog ,neural cell adhesion molecule ( NCAM) ,as well as the neural stem cell markers Nestin and CD 133. Conclusion : The method combining anti-adherent with agarose and NSCs culturing meclium can induce the differentiation of adult human BMSCs into neurospheres with NSCs characteristics , and is one promising strategy to provide large quantities of seed cells for repairing neural system injuries.关键词
骨髓间质干细胞/神经干细胞/巢蛋白/CD133/神经细胞黏附分子/音猬因子/琼脂糖分类
医药卫生引用本文复制引用
庄琴,朱彦,陆益龙,唐爱国,宗海洋,贺清华,陈谦,姜平,张志坚..成人骨髓间质干细胞向神经干细胞的诱导分化[J].江苏大学学报（医学版）,2011,21(2):93-96,封2,5.基金项目
国家自然科学基金资助项目(30570981) （30570981）
