临床与实验病理学杂志2011，Vol.27Issue(2)：126-129,4.
利用FISH技术检测胃癌组织中HER2基因及其与p53蛋白表达的相关性
Use of FISH in detection of HER2 gene and its relationship with p53 protein expression in gastric carcinoma
王仰坤 1高春芳 1张现伟 1贠田 1李富林 1赵元珍 1赵文召 1蒙念龙1
作者信息
- 1. 解放军第150中心医院病理科/济南军区医学病理中心,洛阳,471031
- 折叠
摘要
Abstract
Purpose To investigate the significance of HER2 gene amplification with p53 protein expression and their relationship in gastric cancer. Methods Using FISH and immunohistochemistry techniques, HER2 gene status and p53 protein was detected in 67 cases of gastric cancer. Results HER2 gene amplification rate was 17. 9％ ( 12/67 ) ,HER2 gene amplification in 55 cases without copy number and gene amplification was 49. 3％( 33/67 ). HER2 protein expression rate was 43. 3％( 29/67 ),ratio of HER2 gene amplification to the HER2 protein expression of 3 ＋ cases and 2 ＋ cases was 3/4 and 6/9 .while 3/16 in 1 ＋ cases , with statistically significant difference( P ＜ 0. 05 ). p53 expression rate was 58. 2％( 39/67 ),HER2 gene amplification and p53 protein expression rates were significantly correlated with the infiltration depth of gastric cancer P ＜ 0. 05 ) ,lymphatic metastasis( P ＜ 0. 05 ) . hut not with histology type ( P ＞0. 05 ),age at onset( P ＞ 0. 05 ) , and gender( P ＞ 0. 05 ). Conclusions Rate of HER2 gene amplification, increase of the gene copy number and p53 protein expression are positively correlated, and their combination can be used to predict the metastasis of gastric cancer, and to evaluate the patient' s prognosis , which plays an important role in the individualized treatment.关键词
胃肿瘤/HER2基因/p53蛋白/FISH/免疫组织化学/预后分类
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王仰坤,高春芳,张现伟,贠田,李富林,赵元珍,赵文召,蒙念龙..利用FISH技术检测胃癌组织中HER2基因及其与p53蛋白表达的相关性[J].临床与实验病理学杂志,2011,27(2):126-129,4.
