广东医学2011，Vol.32Issue(1)：29-31,3.
NF-κB圈套寡脱氧核苷酸联合紫杉醇对肺癌血管生成的影响
Effects of NF-κB decoy oligonucleotide combined with paclitaxel on angiogenesis in lung cancer
摘要
Abstract
Objective To investigate the effects of NF - κB decoy oligodeoxynucleotide technology ( NF - κB decoy ODN ) combined with paclitaxel on angiogenesis in lung cancer. Methods Human lung cancer cell A549 was cultured. Full phosphorothioate NF - κB decoy Oligonucleotides ( decoy ODN ) and all missense decoy phosphorothioate oligonucleotides ( scramble decoy ODN ) were transfected with liposome - mediated technique into A549 cells. Treated A549 cells were assigned into control group, NF -κB decoy ODN group, paclitaxel group and NF - κB decoy ODN plus paclitaxel group. RT - PCR and immunohistochemical methods were applied to measure cyclooxygenase -2 ( COX -2 ) mRNA expression level and vascular endothelial growth factor ( VEGF ) protein expression. Results Either NF - κB decoy ODN or paclitaxel intervention had significantly lowered both COX - 2 mRNA and VEGF protein expression in A549 cells ( P ＜ 0. 05 ).Futhermore , expression levels of COX - 2 mRNA in each group were ( 0. 71 ± 0. 04 ) μg/μL、( 0. 44 + 0. 08 ) μg/μL、( 0. 51 ± 0. 02 ) μg/μL、( 0. 34 ±0. 05 ) μg/μL, respectively ( P ＜0. 05 ), while the VEGF positive rates were ( 85 ±0. 50 )％ 、( 63 ± 0. 26 )％ 、 ( 57 ± 1. 5 )％ 、( 21 ±0. 34 )％, respectively ( P ＜0. 05 ). Conclusion NF - κB decoy ODN plus paclitaxel treatment inhibits angiogenesis in lung cancer, which is related to reduced lowering of COX - 2 and VEGF expression.关键词
肺癌/NF-κB圈套寡脱氧核苷酸/紫杉醇/环氧化酶-2/血管内皮生长因子引用本文复制引用
王宁,邢丽华,高炜,高景,焦婧,赵世龙..NF-κB圈套寡脱氧核苷酸联合紫杉醇对肺癌血管生成的影响[J].广东医学,2011,32(1):29-31,3.基金项目
河南省教育厅医学科技攻关计划项目(编号:2009A320041) （编号:2009A320041）
