农业环境科学学报2011，Vol.30Issue(3)：468-475,8.
铅锌矿区重金属污染对微生物数量及放线菌群落结构的影响
Response of Microbe Quantity and Actinomycetes Community of Heavy Metal Contaminated Soils in Leadzinc Mine
摘要
Abstract
To understand the influence of the heavy metal pollution on actinomycetes, the microbe quantity of soils, diversity and dominant microbial groups of actinomycetes were tested.Forty-three strains of actinomycetes in the lead-zinc mine soils were isolated from Fuquan town, Hanyuan country, Sichuan Province.The genetic diversity was evaluated by BOXAIR and 16S rDNA PCR-RFLP.The results indicated that heavy metals pollution had a significant impact on the number of soil microbes in lead-zinc district.With the intensification of heavy metal pollution levels, the total number of soil microorganisms declined.There was a very significant negative correlation between the number of bacteria and heavy metal content(P＜0.01 ), whereas significant negative correlation for the numher of fungi and actinomycetes(P＜0.05 ).The strains used in numerical taxonomy were analyzed by 16S rDNA PCR-RFLP with three kinds of restriction eadonucleases (Hae Ⅲ, Hinf Ⅰ and Taq Ⅰ ) and had 32 genotypes.Based on the dendrograms generated by BOXAIR-PCR, the strains were divided into 10 groups at the similarity of 86％.In addition, BOXAIR-PCR and 16S rDNA PCR-RFLP clustering analysis showed that the strains isolated from soil sampies with the high content of heavy metal clustered together.It could be concluded that Streptomyces was the dominant genus of actinomycetes at the lead-zinc mine in Hanyuan country and had high genetic diversity.关键词
铅锌矿区/微生物数量/放线菌/BOX-AIR/16S rDNA PCR-RFLPKey words
lead-zinc mine/ microbe quantity/ actinomycetes/ BOX-AIR/ 16S rDNA PCR-RFLP分类
资源环境引用本文复制引用
李小林,颜森,张小平,韦成健..铅锌矿区重金属污染对微生物数量及放线菌群落结构的影响[J].农业环境科学学报,2011,30(3):468-475,8.基金项目
国家自然科学基金资助项目(31070004) （31070004）
四川农业大学"双支"计划项目(01470104) （01470104）
