江苏大学学报（医学版）2011，Vol.21Issue(2)：127-130,封2,5.
外源性一氧化碳释放分子2对脓毒症大鼠肺炎症反应的抑制作用及分子机制
Carbon monoxide-releasing molecule-2 inhibits the inflammatory response in the lung of sepsis rat
摘要
Abstract
Objective: To investigate the anti-inflammatory effects of carbon monoxide -releasing molecules-2 ( CORM-2) on lung inflammation in sepsis rat. Methods : Twenty-four Sprague Dawley rats were randomly divided into 3 groups : ①sham group , ②cecal ligation and puncture ( CLP) group and ③CLP + CORM-2 group. To make standard cecal ligation and puncture models , exogenous carbon monoxide was intervened. After 48 h of CLP operation , their pathological changes of lung tissues observed and the expression of TNF-cα 、 IL-1 β and IL-6 in plasma samples was detected respectively by radioimmunoassay ; Level of intercellular adhesion molecule -1 ( ICAM-1 ) in the lung was assessed by ELISA ; Expression of NF-κB was assessed by Western-blot. Results: In CLP group the permeability of lung tissues increased , the alveolar wall turned thick , with interstitial edema and Europhiles infiltrated increasingly ; the expression levels of inflammatory factors and ICAM -1 enhanced obviously , this was accompanied by an increase of the expression of NF-κB. Compared with CLP group, the permeability of lung tissues in CORM -2 group markedly decreased , the alveolar wall was less thicker , interstitial edema and Europhiles infiltration were all alleviated ;the expression levels of inflammatory factors decreased apparently , the level of ICAM-1 in the lung and expression of NF-κB were decreased obviously. Conclusion : CORM-released CO attenuates the inflammatoryresponse in the lung of CLP rats by anti -NF-κB.关键词
一氧化碳释放分子2/脓毒症/炎症/核因子-κB分类
医药卫生引用本文复制引用
袁菊芳,张赢予,濮剑峰,邵东华,孙炳伟..外源性一氧化碳释放分子2对脓毒症大鼠肺炎症反应的抑制作用及分子机制[J].江苏大学学报（医学版）,2011,21(2):127-130,封2,5.基金项目
国家自然科学基金资助项目(30772256 （）
81071546) （）
江苏大学临床医学科技发展基金资助项目(JLY20080052) （JLY20080052）
