广东医学2011，Vol.32Issue(2)：178-181,4.
TLR4和NF-κB及细胞因子炎症反应在急性胰腺炎发病中的作用
Role of TLR4, NF -κB and cytokine - associated inflammation reaction in acute pancreatitis.
摘要
Abstract
Objective To study cascade release of inflammation mediators with activation of NF - κB by TLR4 and its clinical significance in acute pancreatitis. Methods Expression of NF - κB in peripheral neutropils and TLR4 in peripheral monocytes was evaluated at 24th, 72nd and 168th h in MAP and SAP using electrophoresis mobility shift assay and flow cytometry, respectively. Meanwhile, blood TNF -α, IL -1β and IL - 6 were assessed. Correlation analysis was performed among groups. Results The expression of TLR4 and activation level of NF - κB were significantly increased in MAP and SAP groups, compared with those in control groups at 24th h, with more prominent increase of TLR4 expression in SAP group. These elevations descended gradually with the prolongation of time. It was revealed that the activation of NF - κB in PMN and the expression of TLR4 on PBMCs were significantly positively correlated with APACHE - Ⅱ scores and levels of TNF - α and IL - 1 β, and descended following a time - depend manner, during the first week in both SAP and MAP groups according to Pearson correlative analysis. However, no significant correlation with IL -6 was revealed.Conclusion The research dynamically and comprehensively shows that TLR4 causes profuse release of inflammation mediators by activating NF -κB in pathogenesis of human acute pancreatitis, which will exacerbate the condition, thus provides evidence for prognosis predicting, prevention and treatment of acute pancreatitis in the future.关键词
核因子-κB/Toll样受体4/急性胰腺炎Key words
NF -κB/ TLR4/ acute pancreatitis引用本文复制引用
尹金柱,周季英,郑超伟,程明,于丰彦,曾育辉,梁秋玲,潘敏,陈瑞芳,蔡洁毅,袁瑜,邝小枫..TLR4和NF-κB及细胞因子炎症反应在急性胰腺炎发病中的作用[J].广东医学,2011,32(2):178-181,4.基金项目
广东省医学科学技术研究基金项目(编号:WSTJJ20080108320106196803022031) （编号:WSTJJ20080108320106196803022031）
