中医药导报2011，Vol.17Issue(3)：82-84,3.
昆明山海棠总生物碱对LPS诱导的小鼠RAW264.7细胞分泌TNF-αNO的影响
Effects of Total alkaloids of Tripterygium Hypoglaucum (Levi) Hutch on the production of TNF-a and NO by LPS-induced RAW264.7 cells
姜晓 1敖林 1崔志鸿 1黄晓春 1曹佳 1刘晋祎
1
作者信息
- 1. 第三军医大学军事预防医学院军事毒理学教研室,重庆,400038
- 折叠
摘要
Abstract
Objective To investigate the anti-inflammatory mechanism of THHta on LPS-induced RAW264.7 cells. Methods The cytotoxicity of THHta on RAW264.7 cells was detected by CCK8 method. Inflammatory cellular model was established by lipopolysaccharide (LPS) interfering on RAW264.7 cell line. Levels of TNF-α and NO in supernatant were assayed by ELISA or Nitrate acid reductase method. Result THHta inhibited the secretion of TNF-α and NO in LPS-induced RAW264.7cells. Conclusion The anti-inflammatory effects of THHta may be mediated in part by inhibiting the production of inflammatory mediators.关键词
昆明山海棠/总生物碱/脂多糖/TNF-α/NO分类
医药卫生引用本文复制引用
姜晓,敖林,崔志鸿,黄晓春,曹佳,刘晋祎
..昆明山海棠总生物碱对LPS诱导的小鼠RAW264.7细胞分泌TNF-αNO的影响[J].中医药导报,2011,17(3):82-84,3.
