中医药导报2011，Vol.17Issue(3)：87-89,3.
广枣总黄酮对CVB3病毒引起细胞凋亡的影响
The effect of TFC to cell apoptosis induced by CVB3 virus ABSTRACT
刘爽1
作者信息
- 1. 内蒙古医学院第二附属医院,内蒙古,呼和浩特,010000
- 折叠
摘要
Abstract
To establish model of viral myocarditis in vitro, To distill TFC(Total flavones of choerospondias axillaries fructus) with a new method, To test the anti-viral effect of TFC. Method: Using chemical means to distill TFC and test content with HPLC method. TCID50 of CVB3 (coxsackievirusB3) was determined with Reed-Muench method,the anti-viral effect of TFC in vitro was validated through cytopathic effect experiment. Cardiomyocyte Apoptosis was performed by using Hoechst33258、Flow cytometry. Result: The viral myocarditis model in vitro was successfully estahlished,TFC can inhibit CPE (P〈0.05 )in CPE experiment.Virus-reproducing inhibiting experiment shows TFC can effectively inhibit coxsackievirus , Logarithm Value raise more than one unit,Apoptosis rate of cells was reduced. Conclusion: we established viral mycarditis model in vitro and proved TFC is an effective antiviral drug, TFC can reduce the rate of apoptosis induced hy CVB3.关键词
广枣总黄酮/病毒性心肌炎/CVB3/细胞凋亡分类
医药卫生引用本文复制引用
刘爽..广枣总黄酮对CVB3病毒引起细胞凋亡的影响[J].中医药导报,2011,17(3):87-89,3.
