实用肝脏病杂志2011，Vol.14Issue(2)：91-94,4.DOI:10.3969/j.issn.1672-5069.2011.02.002
siRNA沉默β-Catenin对肝星状细胞增殖和凋亡的影响
Effect of interfering RNA targeting β-Catenin on the proliferation and apoptosis of hepatic stellate cells in vitro
摘要
Abstract
Objective To investigate the effect of synthetic small interfering RNA (siRNA)targeting β-Catenin on the biological characters of hepatic stellate cells (HSCs)in vitro. Methods Synthetic siRNA targeting β -Catenin was transfected into HSC-T6 cells by lipofectamine package. Negative siRNA transfection and no transfection were used as negative and blank control,respectively. After incubation with siRNA,total RNA and protein of HSC-T6 cells were extracted. The expression of β-Catenin gene and protein was detected by reverse transcription-polymerase chain reaction and Western blotting. The proliferation of HSC-T6 was determined using MTT assay. HSC-T6 proliferation and apoptosis were measured by flow cytometry. Content of collagen type Ⅰ and Ⅲ in the supernatants were determined by Western blotting. Results After HSC-T6 cells transf'ected with different concentrations of β-Catenin SiRNA,the level of β-Catenin mRNA decreased significantly as compared to the negative and blank contro1;β-Catenin protein expression was inhihited by 56.43±2.88％(P＜0.01),and Ⅰ and Ⅲ collagen expression were also inhibited by 46.58±3.46％(P＜0.01)and 50.48±3.72％(P＜0.01);the cell proliferation was significantly inhibited(P＜0.01).while cell apoptosis rate was significantly higher than in the negative control (P＜0.05). Conclusion Inhibition of β-Catenin by siRNA may have a potential effect on prevention and treatment of hepatic fibrosis by inhibiting proliferation of and promoting apoptosis of stellate cells.关键词
肝星状细胞/小分子干扰RNA/β-Catenin基因/增殖/凋亡引用本文复制引用
葛文松,吴建新,陈颖伟,范建高..siRNA沉默β-Catenin对肝星状细胞增殖和凋亡的影响[J].实用肝脏病杂志,2011,14(2):91-94,4.基金项目
上海市教委选拔培养优秀青年教师科研专项基金(编号:jdy08086) （编号:jdy08086）
