实用医学杂志2011，Vol.27Issue(5)：773-775,3.DOI:10.3969/j.issn.1006-5725.2011.05.017
异位妊娠特征性代谢组学的研究
Study of specific metabonomics of ectopic pregnancy
摘要
Abstract
Objective To investigate the specific metabolites of ectopic pregnancy by using ultraperformance liquid chromatography coupled with Q Time-Of-Flight mass spectrometry (UPLC/Q-TOF MS) , and to establish a diagnosis model for ectopic pregnancy. Methods Serum samples of 9 cases with ectopic pregnancy , 15cases with ahortion of intrauterine pregnancy , and 20 cases with normal intrauterine pregnancy were detected by UPLC-Q/TOF MS analysis technique. Principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were made in metabolic finger print data of different groups and specific metabolites of ectopic pregnancy were screened out. Results After the UPLC-Q/TOF MS analysis , the retention time and m/z data pair for each peak were detected. PLS-DA and coefficient of correlation analysis were used for markers selection.According to these data , 13 potential discriminant metabolites of ectopic pregnancy , as compared to intrauterine pregnancy, were found. Conclusion Thirteen ectopic pregnancy related metabolites were found which may be specific for and of significance in the early diagnosis of ectopic pregnancy.关键词
妊娠,异位/代谢组学/诊断引用本文复制引用
蔡美玲,祝亚平,严沁,谢毅,杨烨,姜丽,吴凌,魏亚楠..异位妊娠特征性代谢组学的研究[J].实用医学杂志,2011,27(5):773-775,3.基金项目
上海市卫生局科研课题基金资助项目(编号:2007141) （编号:2007141）
