实用医学杂志2011，Vol.27Issue(6)：938-941,4.DOI:10.3969/j.issn.1006-5725.2011.06.005
重组人pEGFP-C2-NPHS2表达载体的构建及其在人胚胎肾293细胞中的表达
Construction of human recombinant pEGFP-C2-NPHS2 and its expression in human embyo kidney 293 cell lines
摘要
Abstract
Objective To construct the pEGFP-C2-NPHS2 expression vector and ohserve its expression in HEK293 cells. Methods Technique of gene recombination was used to construct the expression vector pECFP-C2-NPHS2. PCR, restriction enzyme digestion, and DNA sequencing were used to identify the correct vector. The recombinant expression vector was transfected into HEK293 cells and detected by fluoroscopy and Western blot.Results The target gene NPHS2 was inserted into the vector pEGFP-C2. Green fluorence located on HEK293 cell membrane.The protein podocin expression of NPHS2 was detected by western blot. Conclusion The pEGFP-C2-NPHS2 expression vector was successfully constructed and would contrihute to the further study on the role of podocin in podocyte disease.关键词
足细胞/HEK293细胞/NPHS2/表达载体/基因重组引用本文复制引用
沙玉根,赵非,张爱华,黄松明..重组人pEGFP-C2-NPHS2表达载体的构建及其在人胚胎肾293细胞中的表达[J].实用医学杂志,2011,27(6):938-941,4.基金项目
国家自然科学基金资助(编号:30100081) （编号:30100081）
南京市卫生局资助项目(编号:ZKM06043) （编号:ZKM06043）
