实用医学杂志2011，Vol.27Issue(5)：733-735,3.DOI:10.3969/j.issn.1006-5725.2011.05.001
干扰素α-2b对HepG 2.2.15细胞增殖、凋亡及HBsAg与HBeAg表达的影响
The influences of interferon α-2b on the proliferation, apoptosis, expression of HBsAg and HBeAg in HepG 2.2.15 cells
摘要
Abstract
Objective To investigate the influences of' interferon α-2b (IFN a-2b) on the proliferation,apoptosis, expression of HBsAg and HBeAg in HepG 2.2.15 cells. Methods After treated with IFN α-2b for 3 days or 6 days, the proliferation and apoptosis of HepG 2.2.15 cells were analyzed by SRB assay and double staining with Hochest 33342 and PI respectively. And the expression of HBsAg and HBeAg in treated HepG 2.2.15 cells were assayed by ELISA. Results IFN α-2b treatment inhibits the proliferation of HepG 2.2.15 cells and causes a G0/CJ, cell cycle arrest. The expression of both HBsAg and HBeAg were inhibited in a dosedependent manner after treated with IFN α-2b for 3 days. And the expression of HBsAg was further downregulated af'ter 6 days, while the expression of HBeAg was similar to that of treated for 3 days. Hochest 33342 and PI double staining revealed that IFN α-2b also induced apoptosis in a concentration dependent manner. Conclusion IFN α-2b treatment inhibited not only the expression of HBsAg and HBeAg , but also the prolif'eration of HepG 2.2.15 cells and induced apoptosis in HepG 2.2.15 cells.关键词
干扰素α-2b/HepG 2.2.15/HBsAg/HBeAg/增殖/凋亡引用本文复制引用
周尧远,蒋孟军,张荣军,蔡刚明,张波,顾晓波,邹美芬..干扰素α-2b对HepG 2.2.15细胞增殖、凋亡及HBsAg与HBeAg表达的影响[J].实用医学杂志,2011,27(5):733-735,3.基金项目
国家自然科学基金项目(编号:30670589) （编号:30670589）
卫生部科研基金项目(编号:wkj2006-2-5) （编号:wkj2006-2-5）
江苏省医学重点人才项目(编号:RC2007097) （编号:RC2007097）
江苏省人事厅"六大人才高峰"项目(编号:06-B-026) （编号:06-B-026）
无锡市"学术技术带头人培养工程"资助项目 （）
