实用医学杂志2011，Vol.27Issue(6)：967-970,4.DOI:10.3969/j.issn.1006-5725.2011.06.017
霉酚酸酯诱导Balb/c小鼠调节性T细胞的分化和增殖
Mycophenolate mofetil promotes differentiation and proliferation of mouse CD4+CD25+ regulatory T cells
摘要
Abstract
Objective To analyze the influence of mycophenolate mofetil on the differentiation and proliferation of CD4+CD25+Treg cells, and reveal its possible role in inducing immune tolerance. Methods Twenty-four 8-week-old SPF Balb/c mice were randomly divided into three groups, mycophenolate mofetil group (MMF) , cyclosporine group (CsA) and sham group. They were intraperitoneally administrated with mycophenolate mofetil 40 mg/(kg · d) , CsA 10 mg/( kg · d) and same volume saline respectively. After three weeks , orbital vein blood was collected and spleen cells suspension were prepared. Flow cytometry was used to determine the proportions of CD4+CD25+Treg cells , and the expression of Foxp3 protein in spleen was analyzed by immunochemistry. Results The CD4+CD25+Treg cells of peripheral and spleen were (6.35 ± 0.09)％ and ( 11.62 ± 1.10)％ in MMF group , (2.57 ± 0.09 ) ％ and (5.46 ± 0.75 ) ％ in CsA group , (4.64 ± 0. 13 )％ and (7.61 ± 0.73 )％ in Sham group respectively.There were significant differences among these three groups (P ＜ 0.01). Immunochemistry analysis showed that compared to the sham group , the expression of Foxp3 protein in CsA group decreased significantly (P ＜ 0.01) ,while its expression in MMF group increased signiricantly (P ＜ 0.01 ). Conclusion MMF promotes the proliferation of CD4+CD25 +Foxp3 +Treg cells in vitro , and facilitates the development of immune tolerance. And its immune suppression mechanism is different from that of cyclosporine A.关键词
T淋巴细胞,调节性/霉酚酸酯/环孢素A/Foxp3/细胞分化/细胞增殖引用本文复制引用
龚淑文,杨梅,张岩,庄垟垟,杨亦荣,郑少玲..霉酚酸酯诱导Balb/c小鼠调节性T细胞的分化和增殖[J].实用医学杂志,2011,27(6):967-970,4.基金项目
浙江省自然科学基金资助项目(编号:Y206924) （编号:Y206924）
