疑难病杂志2011，Vol.10Issue(4)：285-288,封3,5.DOI:10.3969/j.issn.1671-6450.2011.04.017
丰白散对COPD模型大鼠肺组织中MMP-9及TIMP-1表达的影响
Effect of Fengbaisan on the expression of MMP-9 and TIMP-1 in rats with chronic obstructive pulmonary disease
摘要
Abstract
Objective To observe the effect of the traditional Chinese medicine Fengbaisan on the expression of matrix metalloproteinase-9( MMP-9 )and tissue inhibitor of metalloproteinase-l( TIMP-1 ) in rats with chronic obstructive pulmonary disease( COPD ) ,and to investigate the potential mechanisms of the decoction. Methods There were 10 rats enrolled as control group, with another 36 rats induced with COPD model, which was established by modified method of combining fumigation and lipopolysaccharide ( LPS ) intra tracheal dripping. The 30 successful COPD rats were divided into model group, ambroxol treatment group and Fengbaisan treatment group. After 28 days interventional therapy, the lung tissue sections stained by HE were observed to study the morphological changes, the reverse transcription polymerase chain reaction( RT-PCR ) was used to evaluate the mRNA levels of MMP-9 and TIMP-1. Results In control group, the alveolus structure was integrities, and no defect in alveolus septum. The typical pathological changes of emphysema were emerged in three groups of COPD model of rats, included the alveolus structure was ruptured and fusion, and elastic fibers was damaged. The pathological changes were released in ambroxol and Fengbaisan treatment groups. Compared with the normal control group, the expression of MMP-9 mRNA and TIMP-1 mRNA in COPD model group were increased significantly( P ＜0.05 ). The expression of MMP-9 mRNA and TIMP-1 mRNA in the Fenbaisan and ambroxol treatment group were decreased markedly as compared to the COPD model group( P ＜0.05 ), while no statistical difference was identified inter-groups( P ＞0. 05 ). Conclusion The traditional Chinese medicine Fengbaisan decoction could reduce the mRNA levels of MMP-9 and TIMP-1, and correct the MMP-9/TIMP-1 imbalance, which may be one of the mechanisms of the decoction to treat COPD.关键词
丰白散/肺疾病,阻塞性,慢性/基质金属蛋白酶-9/基质金属蛋白酶组织抑制剂-1/大鼠引用本文复制引用
王煜,苏南湘..丰白散对COPD模型大鼠肺组织中MMP-9及TIMP-1表达的影响[J].疑难病杂志,2011,10(4):285-288,封3,5.基金项目
湖南省中医药科研基金资助项目(No. 2008014) （No. 2008014）
