西安交通大学学报（医学版）2011，Vol.32Issue(1)：114-118,127,6.
尾型同源盒基因-2过表达对结肠癌细胞生物学性状的影响
CDX2 gene overexpression and its inhibition on malignant progress of human colorectal cancer cell line
摘要
Abstract
Objective To investigate the effect of human caudal-related homeobox 2 (CDX2) gene expression on human colorectal cancer cell line Lovo and explore CDX2's role in the pathogenesis and development of colorectal cancer. Methods Human colon gene CDX2 was cloned and the pEGFP-C1-CDX2 eukayotic expression plasmid was constructed by DNA recombinant method. Lovo cells were divided into three groups: transfected with pEGFPC1-CDX2, transfected with pEGFP-C1, and non-transfected. We transfected pEGFP-C1-CDX2 and pEGFP-C1 plasmid into Lovo cells separately with lipofectamine 2000. CDX2 gene expression in Lovo cells was detected by Western blotting. The effects of CDX2 overexpression on the proliferation, apoptosis and cycle of Lovo cells were assessed by MTT and flow cytometry. The effects on the matrix metalloproteinase-2 (MMP-2) expression were detected by ELISA method. Results pEGFP-C1-CDX2 eukayotic expression plasmid was constructed successfully and there was a higher expression of CDX2 protein in Lovo cells which had transient transfection of pEGFP-C1CDX2 plasmid for 48 hours. Compared with that of the cells transfected with empty vector or non-transfected cells, the growth of cells transfected with pEGFP-C1-CDX2 was not inhibited obviously after 72 hours (P＞0.05). The proportion of G1 and S phases of cell cycle progression did not change obviously (P＞0.05). Apoptosis rate was not increased obviously, either ( P ＞ 0.05). However, the secretion of MMP-2 was reduced obviously ( P ＜ 0.05). Conclusion The overexpression of CDX2 gene has no obvious effect on the proliferation, apoptosis or cycle of Lovo cells, but it can significantly weaken the invasiveness and migration of Lovo cells; therefore, it may play an important role in inhibiting the invasion and metastasis of colorectal cancer.关键词
尾型同源盒基因-2/结直肠癌/增殖/凋亡/周期/基质金属蛋白酶-2分类
医药卫生引用本文复制引用
李守帅,孙学军,郑见宝,任海亮..尾型同源盒基因-2过表达对结肠癌细胞生物学性状的影响[J].西安交通大学学报（医学版）,2011,32(1):114-118,127,6.基金项目
国家自然科学基金资助项目(No.30672070,No.30400430) （No.30672070,No.30400430）
陕西省科学技术研究发展计划资助项目(No.2009K12-01) （No.2009K12-01）
