浙江医学2011，Vol.33Issue(3)：334-336,340,4.
姜黄素对哮喘大鼠IL-5、Eotaxin 水平及NF-κB表达的影响
Effects of curcumin on IL-5 level and expression of NF-κB and eotaxin in asthmatic rats
摘要
Abstract
Objective To investigate the effects of curcumin on IL- 5 levels in serum and bronchoalveolar lavage fluid (BALF), and expression ofNF- κ B and eotaxin in lung tissue of asthmatic rats Methods Thirty six rats were randomly divided into three groups: normal control group (group A), asthma group (group B), curcumin treatment group (group C). The asthma model was induced by ovalbumin (OVA) challenge. The concentrations ofIL- 5 in serum and BALF were measured by sandwich ELISA,EOS cells in BALF were counted,the expressions ofNF- κ B p65 and eotaxin were determined by immunehistochemistry techniques. Results The concentrations of IL-5 in serum and BALF of group B was significantly higher than those of group A and group C (P＜0.01). Immunohistochemistry showed that the expression of NF- κ B p65 of was in cytoplasm and nuclei of epithelial cells and inflammatory cells. The expression of NF-κ BP65 in group B was significantly higher than those of group A and group C (P＜0.01). Immunohistochemistry showed that eotaxin was mainly in cytoplasm of the bronchus epithelialcells. The expression of Eotaxin in group B was significantly higher than those of group A and group C (P＜ 0.01). The expression of NF- κ B p65 in lung tissue was positively correlated with EOC count, IL- 5 level and eotaxin expression (r=0.928, 0.905, 0.850,allP＜0.01). Conclusion Curcumin can significantly down- regulate NF- κ B and in turns inhibit IL- 5 and eotaxin expression in asthmatic rats.关键词
姜黄素/支气管哮喘/NF-κB/Eotaxin/IL-5引用本文复制引用
黄建达,应骏,顾铜杰..姜黄素对哮喘大鼠IL-5、Eotaxin 水平及NF-κB表达的影响[J].浙江医学,2011,33(3):334-336,340,4.
