中国药理学通报2011，Vol.27Issue(4)：467-472,6.DOI:10.3969/j.issn.1001-1978.2011.04.007
丙酸睾酮对大肠杆菌RecQ解旋酶结构和功能的影响
Effects of testosterone propionate on the structure and function of E.coli RecQ helicase
摘要
Abstract
Aim To study the effects of testosterone propionate on the bioactivity and structure of E. coli RecQ helicase, and the interaction molecular mechanisms in vitro. Methods The effects of the testosterone propionate on the helicase activity , DNA binding activity, ATPase activity, and the structure of E. coli RecQ helicase were assayed by fluorescence polarization , PiColorLock Gold reagent, and ultraviolet aborp-tion spectrum respectively. Results The biological activity and structure of E. coli RecQ helicase were weakly effected by testosterone propionate. The helicase activity was promoted at low oncentration and inhibited at high concentration, the Ki for DNA binding activity was below 7 × 10 nmol· L when sustrate was dsD-NA, but the inhibition was very weak when sustrate was ssDNA, and the Ki at least 7 × 10-3 nmol· L-1 . RecQ helicase UV absorption peak was changed by tes-tosterone propionate, but the maximum UV absorption peak would not deviate. Conclusions The interaction between E. coli RecQ helicase and testosterone propio-nate was weak. The binding sites between testosterone propionate and E. coli RecQ helicase had two varieties.关键词
丙酸睾酮/大肠杆菌/RecQ解旋酶/解旋酶活性/DNA结合活性/ATPase活性/构象分类
医药卫生引用本文复制引用
段丽霞,许厚强,陈祥,骆衡..丙酸睾酮对大肠杆菌RecQ解旋酶结构和功能的影响[J].中国药理学通报,2011,27(4):467-472,6.基金项目
国家重点基础研究发展计划(973计划)资助项目(No 2010CB 534912) （973计划）
教育部博士点基金资助项目(No 200806570003) （No 200806570003）
贵州省优秀人才省长资金资助项目(No 200822) （No 200822）
贵州省研究生创新基金项目(No 省研农2010004) （No 省研农2010004）
