中国药理学通报2011，Vol.27Issue(4)：482-486,5.DOI:10.3969/j.issn.1001-1978.2011.04.010
水杨酸钠对大鼠颞皮层神经元延迟整流钾通道的抑制作用
Inhibition of sodium salicylate on delayed rectifier potassium channels in rat auditory cortex neurons
摘要
Abstract
Aim To understand what role the delayed rectifier potassium channels play in the mechanism of salicylate-inclucecl tinnitus. Methods The effects of salicylate on the delayed rectifier potassium channels in freshly dissociated auditory cortex neurons of rats were studied, using the whole-cell voltage clamp method.Results Salicylate blocked the delayed rectifier potas -sium current ( IK(DR) ) in a concentration-dependent manner ( 0. 1 ～ 1 mmol · L-1 ). The half-inhibition concentration (IC50) values for the blocking action of salicylate on IK(DR) were 2. 13 mmol· L-1. At a concentration of I mmol· L-1 , salicylate significantly shifted the activation curve negatively by 14 mV, shifted inactivation curve of IK(DR) negatively by 17 mV, and delayed its time constant ( τ) of recovery curve from inactivation to 171％ of the value before salicylate application. Conclusion Salicylate inhibits IK(DR) in rat auditory cortex neurons and significantly affects the ac -tivation and inactivation kinetics of the delayed rectifier potassium channels, which could be related to the mechanism of salicylate -induced tinnitus .关键词
水杨酸钠/耳鸣/颞皮层/延迟整流钾通道/膜片钳/大鼠分类
医药卫生引用本文复制引用
刘砚星,李学佩,张海林,徐鸥,王娜,陈雪彦..水杨酸钠对大鼠颞皮层神经元延迟整流钾通道的抑制作用[J].中国药理学通报,2011,27(4):482-486,5.基金项目
国家自然科学基金资助项目(No 30600125,30730031) （No 30600125,30730031）
