中国药理学通报2011，Vol.27Issue(4)：508-511,4.DOI:10.3969/j.issn.1001-1978.2011.04.016
土党参多糖对小鼠脑缺血/再灌注损伤的保护作用
Protective effect of polysaccharides from Campanumoea javanica Bl against cerebral ischemia reperfusion injury in mice
摘要
Abstract
Aim To investigate the effect of polysac -charides from Campanumoea javanica Bl on cerebral ischemical reperfusion injury in mice. Methods KM mice were randomly divided into 7 groups: sham group, model group, shuxuening group ( 100 mg ·kg-1) , nimodipine group (100 mg· kg-1) and polysaccharides high , middle , low close ( 300 , 150 , 75 mg · kg-1) groups. The models were established by ligating bilateral common carotid artery. The neuronal morphology in hippocampus was observed , and the contents of malondialdehyde ( MDA) , nitric oxide ( NO) , acetylcholine ( ACh) , glucose and superoxide dismutase ( SOD) , glutathione ( GSH) , acetyl cholinesterase ( AChE) , and ATPase activity were assayed.Results The polysaccharides coulcl evidently alleviate injuries of neurons in hippocampus CA 1 , reduce the brain tissue content of MDA and NO (P ＜0. 05 or P ＜0. 01 ) ,but have no significant effect on SOD , GSH activity. The middle dose group significantly increased ACh content (P ＜0. 01) , while the low and high dose groups produced no significant effect compared with the model group. Middle and high dose groups significantly decreased AChE activity and increased ATP enzyme ,glucose metabolic activity (P ＜ 0. 05 or P ＜ 0. 01 ).Conclusion Polysaccharides from Campanumoea javanica Bl has a protective effect on cerebral ischemiareperfusion injury , and that may be related to neurotrophic, antioxidant, metabolic control, as well as reduced acetylcholinesterase activity .关键词
土党参/多糖/缺血/再灌注/神经营养因子/抗氧化/乙酰胆碱/能量代谢分类
医药卫生引用本文复制引用
张振东,杨娟,吴兰芳,景永帅,彭梅,杨小生..土党参多糖对小鼠脑缺血/再灌注损伤的保护作用[J].中国药理学通报,2011,27(4):508-511,4.基金项目
国家自然科学基金资助项目(No 30760297) （No 30760297）
国家重点基础研究发展计划(973计划)资助项目(No 2009CB526512) （973计划）
贵州省中药现代化科技产业研究开发专项项目基金(No 黔科合社字[2009]5017号) （No 黔科合社字[2009]5017号）
贵阳市科学技术计划项目基金(No [2010]筑科农合同字第1-中-29号) （No [2010]筑科农合同字第1-中-29号）
