中国药理学通报2011，Vol.27Issue(4)：537-542,6.DOI:10.3969/j.issn.1001-1978.2011.04.023
黄芩总黄酮对博莱霉素致大鼠肺纤维化的干预作用
Effects of total flavonoids of scutellaria baicalensis georgi(TFSB)on bleomycin-induced pulmonary fibrosis in rats
摘要
Abstract
Aim To study the effects of total flavonoids of scutellaria baicalensis georgi ( TFSB ) on bleomycininduced pulmonary fibrosis in rats and investigate its mechanisms. Methods The pulmonary fibrosis model was estalished by intratracheal injection of Bleomycin.After 28 d, rats were treated with TFSB by intragastric administration , and the pathomorphology , the biochemistrical index in lung tissue and bloocl were measured and analyzed. The effects of TFSB on the expressions of transforming growth factor-β1 ( TCF-β1 ) , Smad2/3 mRNA and the expressions of alpha-smooth muscle actin ( α -SMA ) and peroxisome proliferator -activated receptor-gamma( PPAR-γ) protein in lung tissue of rats were assayed by RT-PCR and Western blot. Results Compared with the model group , the contents of MDA in serum and lung tissue and the contents of HYP were decreased ( P ＜ 0. 05 , P ＜ 0. 01 ) , the contents of GSH , TAOC and SOD in serum were mcreased ( P ＜ 0. 05 ,P ＜ 0. 01) , the contents of CSH in lung tissue were increased ( P ＜ 0. 01 ) . TFSB could obviously reduce alveolitis and pulmonary fibrosis ( P ＜ 0. 05 ,P ＜ 0. 01) . The expressions of TCF-β1 and Smad2/3 mRNA in lung tissue of rats were decreased ( P ＜ 0. 05 ) , and the expression of α-SMA in lung tissue of rats was decreased ( P ＜0. 05). In contrast, the expression of PPAR -γ protein in lung tissue of rats were increased ( P ＜ 0. 05 ) .Conclusion TFSB has therapeutic effects of bleomycin-induced pulmonary fibrosis in rats , which might be related with the anti-oxidation and the inhibition of TGF-β1 signaling pathway .关键词
肺纤维化/黄芩总黄酮/博莱霉素/转化生长因子-β1/大鼠/过氧化物酶体增殖因子活化受体γ分类
医药卫生引用本文复制引用
马荣林,王尉平,蒋小岗,顾振纶,崔红霞,郭次仪,周文轩..黄芩总黄酮对博莱霉素致大鼠肺纤维化的干预作用[J].中国药理学通报,2011,27(4):537-542,6.基金项目
江苏省中医药管理局项目(No HZ8636620) （No HZ8636620）
香港保健协会研究项目(No 20070909-1,HK) （No 20070909-1,HK）
苏州市科技计划项目(No 苏科计[2008]142号,苏财科字[2008]47号) （No 苏科计[2008]142号,苏财科字[2008]47号）
