中国病理生理杂志2011，Vol.27Issue(2)：234-237,4.DOI:10.3969/j.issn.1000-4718.2011.02-005
内脂素对THP-1源性巨噬细胞ATP结合盒转运蛋白A1表达的影响
Effect of visfatin on expression of ATP binding cassette transporter A1 in THP-1-derived macrophages
摘要
Abstract
AIM : To explore the mechanism of foam cell formation hy investigating the expression of ATP binding cassette transporter A1( ABCA1 ) in human acute monoc.ytic leukemia cell line( THP - 1 ) - derived macrophages treated with visfatin.METHODS : THP - 1 monocytes were induced into macrophages by exposure of the cells to phorbol myristate acetate( PMA, 160 nmol/L ) for 48 h.The macrophages were then treated with visfatin at different concentrations and for different time points.The lipid droplets in cytoplasm of the macrophages were observed by oil red 0 staining.The expression of ABCA1 at mRNA and protein levels was determined by reverse transcriptase polymerase chain reaction( RT - PCR )and Western blotting, respectively.The contents of intracellular total cholesterol( TC ) and free cholesterol( FC ) was measured by enzyme fluorescence analysis.The content of cholesterol ester ( CE ) was calculated according to the difference between TC and FC.RESULTS: Compared with control group, the lipid droplets and the content of FC and CE were increased in visfatin - treated macrophages.The expression of ABCA1 at mRNA and protein levels was down - regulated by visfatin in a concentration - and time - dependent manner ( P ＜ 0.05 ).CONCLUSION : The down - regulation of ABCA1expression is induced by visfatin through reducing FC efflux and increasing CE synthesis.This mav induce the formation of THP - 1 - derived foam cells.关键词
内脂素/ATP结合盒转运体A1/泡沫细胞分类
医药卫生引用本文复制引用
康静,成蓓,姜蕾..内脂素对THP-1源性巨噬细胞ATP结合盒转运蛋白A1表达的影响[J].中国病理生理杂志,2011,27(2):234-237,4.基金项目
国家自然科学基金资助项目(No.30471921) （No.30471921）
