中国药理学通报2011，Vol.27Issue(4)：524-527,4.DOI:10.3969/j.issn.1001-1978.2011.04.020
姜黄素预处理对大鼠脑缺血/再灌注损伤后AQP-4及脑水肿的影响
Effects of chemical synthesized curcumin preconditioning on the expression of AQP-4 and cerebral edema after focal cerebral ischemia/reperfusion damage in rats
摘要
Abstract
Aim To explore the effect of chemical synthesized curcumin ( Cur) preconditioning on the expression of aquaporin 4 ( AQP-4 ) and cerebral edema after focal cerebral ischemia-reperfusion damage in rats. Methods Local ischemia/reperfusion model of middle cerebral artery occlusion ( MCAO) was made by inserting a nylon thread into internal caroticl artery in rats. 72 healthy SD rats weighing 290 ～310 g were randomly divided into 3 groups: sham operated group ( S) ( n = 18) , ischemia/reperfusion group (Ⅰ) ( n =18) , Cur group ( C1 and C2) (n = 18) which were preconditioned with cur 50 or 100 mg· kg-1 intraperitoneally 30 min before focal cerebral ischemia -reperfusion. In Ⅰ group , NS was given instead of Cur and in S group, the carotid arteries were exposed without performing MCAO. The rats in Ⅰ group ancl C1, C2 group were killed 24 hours after cerebral ischemia/reperfusion. Neurological scores were studied , method of dry/wet weight was used to observe water content , and Evans Blue( EB) was collected to observe the changes of blood-brain barrier ( BBB) permeability. Expression of AQP-4 ancl activation of c-Jun N-terminal kinase ( JNK) in rat brain were determined by Western blot.Results Sham operated group presented no neurological changes. The water content and EB in C1 and C2 group were significantly lower than those of Ⅰ group ( P ＜ 0. 05 ) . Curcumin could also alleviate the expression of AQP-4 and the phosphorylation of JNK (P ＜ 0. 05 ) . The neurological deficiency scores in C1 and C2 groups were obviously higher than those of I group ( P ＜ 0. 05 ) . Conclusions Chemical synthesized curcurmn can alleviate the expression of AQP-4 and the phosphorylation of JNK , therefore protect the structure of BBB and alleviate the cerebral eclema which can exert the neuroprotective effect after focal cerebral ischemia ancl reperfusion.关键词
化学合成/姜黄素/脑缺血/再灌注损伤/AQP-4/c-Jun氨基端激酶/血脑屏障分类
医药卫生引用本文复制引用
纪风涛,曹铭辉,梁建军,刘玲,李峰,卜宪章..姜黄素预处理对大鼠脑缺血/再灌注损伤后AQP-4及脑水肿的影响[J].中国药理学通报,2011,27(4):524-527,4.基金项目
广东省科技计划资助项目(No 2010B080701004) （No 2010B080701004）
广东省医学科研基金资助项目(No B2010092) （No B2010092）
