中国病理生理杂志2011，Vol.27Issue(2)：249-253,5.DOI:10.3969/j.issn.1000-4718.2011.02-008
丹皮酚通过抑制NF-κB信号通路下调高脂血清诱导的人脐静脉内皮细胞黏附分子的表达
Paeonol reduces expression of adhesion molecules in HUVECs induced by hyperlipidemic serum via inhibiting the pathway of NF-κB signaling
摘要
Abstract
AIM : To ohserve the anti - atherosclerosis effect of paeonal( Pae ) on the activation of NF - κB and the expression of cell adhesion molecules in human umbilical vein endothelial cells( HUVECs ) induced hy hyperlipidemic serum.METHODS : Cultured HUVECs were used as target cells.Hyperlipidemic serum was added to the culture medium to establish the injury mode of HUVECs.Methyl thiazolyl tetrazolium( MTT ) method was used to examine the cell viability.The mRNA expression of NF - κB p65 was determined hy RT - PCR.The protein levels of IκB - α , intercellular cell adhesion molec:ule - 1( ICAM - 1 )and E - selectin were detected by Western blotting.RESULTS : After treated with Pae , the cell viability was increased and the morphological changes of HUVECs injured by hyperlipidemic serum trended to normal.The expression of IκB - α in HUVECs injured hy hyperlipidemic serum increased, while the expression of NF - κB p65mRNA.ICAM - 1 and E - selectin protein was decreased.CONCLUSION : The anti - atherosclerosis mechanism of paeonal may be related to the inhibitory effect of the natural compound on the pathway of NF - κB/IκB, thereby reducing the expression of ICAM - 1 and E - selectin and attenuating the inflammatory reaction in vascellum.关键词
丹皮酚/高脂血清/脐静脉内皮细胞/NF-κB/胞间黏附分子1/E-选择素分类
医药卫生引用本文复制引用
周晓慧,牛成伟,曹凯,徐倩..丹皮酚通过抑制NF-κB信号通路下调高脂血清诱导的人脐静脉内皮细胞黏附分子的表达[J].中国病理生理杂志,2011,27(2):249-253,5.基金项目
河北省科技厅基金资助项目(No.07276101D-47) （No.07276101D-47）
