中国病理生理杂志2011，Vol.27Issue(2)：357-360,4.DOI:10.3969/j.issn.1000-4718.2011.02-027
siRNA沉默LBH基因促进人支气管上皮细胞周期进展
RNAi-mediated knocking-down of LBH gene promotes cell cycle progress in 16HBE cells
摘要
Abstract
AIM : To investigate the effect of small interfering RNA( siRNA ) on limb - hud and heart( LBH )gene expression in 16HBE cells, and further to observe the change of 16HBE cell cycle after knocking down of LBH gene.METHODS: Synthetical siRNA targeting LBH gene was transfected into 16HBE cells by the method of lipofectamine 2000.The mRNA expression of LBH was examined by real time RT - PCR.The cell cycle was assayed by flow cytometry.The expression of cyclin E1 and E2 was also detected by real time RT - PCR and Western blotting.RESULTS: The expression of LBH gene decreased by 86％ in 16HBE cells transfected with 50 nmol/L of siRNA for 48 h.After transfected with siRNA for 48 h, 16HBE cells in G1 phase decreased by 9.28％ and the cells in S phase increased by 14.08％ .The expression level of cyclin E2 in 16HBE cells transfected with siRNA was twice higher than that of the negative control cells.CONCLUSION: Sequence - specific siRNA targeting LBH is capable of suppressing LBH gene expression in 16HBE cells.Down - regulation of LBH expression in 16HBE cells may promote the progress of cell cycle , and up - regulation of cyclin E2 expression plays a role in the process of G1/S phase.关键词
RNA干扰/基因,LBH/16HBE细胞/细胞周期分类
医药卫生引用本文复制引用
刘启才,王颖峰,杨春祥,李晓艳,彭燕,彭杰,李冰,李立..siRNA沉默LBH基因促进人支气管上皮细胞周期进展[J].中国病理生理杂志,2011,27(2):357-360,4.基金项目
广东省自然科学基金资助项目(No.06301124) （No.06301124）
广州市属高校科技计划资助项目(No.61093) （No.61093）
国家自然科学基金资助项目(No.81071207) （No.81071207）
