中国病理生理杂志2011，Vol.27Issue(2)：378-381,385,5.DOI:10.3969/j.issn.1000-4718.2011.02-032
二十碳五烯酸对脂多糖处理的人脐静脉内皮细胞核转录因子κB的表达及细胞因子分泌的影响
Effects of eicosapentaenoic acid on expression of nuclear factor κB and release of cytokines in human umbilical vein endothelial cells stimulated by lipopolysaccharide
包永琴 1马景学 1叶存喜 1董白霞 1翟英1
作者信息
- 1. 河北医科大学第二医院眼科,河北,石家庄,050000
- 折叠
摘要
Abstract
AIM : To investigate the effects of eicosapentaenoic acid( EPA ) on the expression of nuclear factor kappa B( NF - κB ) and release of vascular endothelial growth factor( VEGF ),IL - 1α and IL - 6 in cultured human umbilical vein endothelial cells( HUVECs ) stimulated by lipopolysaccharide( LPS ).METHODS: HUVECs were ohtained from cell strain and cultured in vitro.HUVECs were divided into 4 groups: control group, LPS group, 0.030 g/L EPA treatment group and 0.050 g/L EPA treatment group.The cells were cultured with LPS alone in LPS group and incubated with EPA for 1 h in the EPA pretreatment groups at the concentrations of 0.030 gL and 0.050 g/L before LPS stimulation.Twenty - four hours after stimulated by LPS, the protein expression of NF - κB p65 in HUVECs were assessed by Western blotting analysis at different time points.The production of VEGF, IL - 1α and IL - 6 in cultured HUVECs was evaluated by ELISA.The effects of EPA on the protein expression of NF - κB p65 and the production of VEGF, IL - 1α and IL -6in HUVECs challenged by LPS were also determined.RESULTS : Compared with control group, the protein expression of NF - κB p65 was significantly increased in HUVECs induced by LPS and was inhibited by EPA.Compared with control group, the protein expression of VEGF, IL - 1α and IL -6 was dramatically increased in HUVECs induced by LPS and most of the increase was inhibited hy EPA.CONCLUSION : LPS enhances the protein expression of NF - κB and the release of VEGF, IL - 1α and IL -6.EPA inhibits the protein expression of NF - κB, and the production of VEGF and the inflammatory cytokines in cultured HUVECs stimulated by LPS, indicating that EPA may be useful for preventing and treating neovascular and inflammatorv diseases.关键词
二十碳五烯酸/人脐静脉内皮细胞/NF-κB/血管内皮生长因子/细胞因子类分类
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包永琴,马景学,叶存喜,董白霞,翟英..二十碳五烯酸对脂多糖处理的人脐静脉内皮细胞核转录因子κB的表达及细胞因子分泌的影响[J].中国病理生理杂志,2011,27(2):378-381,385,5.
