中国病理生理杂志2011，Vol.27Issue(2)：412-416,5.DOI:10.3969/j.issn.1000-4718.2011.02-040
大鼠活体心肌缺血再灌注损伤模型的建立与综合评估
Establishment and evaluation of rat heart ischemia-reperfusion injury model in vivo
摘要
Abstract
AIM: To estahlish and evaluate a rat model of heart ischemia - reperfusion injury in vivo.METHODS : Seventy - two male Sprague - Dawley rats weighing( 250 ±50 )g were randomly divided into sham operation group( sham ), ischemia - reperfusion group( I/R ) and normal group.The animals were anesthetized and heparinized.Myocardial ischemia - reperfusion was induced by ligating the left anterior descending coronary artery with " U - shape tube" for 35 min followed hy 120 min or 240 min reperfusion in vivo.The heart infarct size was measured by triphenyltetrazolium chloride( TTC ) staining.The myocardial cell apoptotic index was determined by the method of terminal deoxynucleotidyl transferase - mediated dUTP - biotin nick - end labeling( TUNEL ).Immunohistochemical method was used to detect the expression of Bcl - 2 and Bax in rat ischemia myocardium.The blood level of MB isoenzyme of creatine kinase ( CK - MB ), cardiac troponin Ⅰ( cTnI ), nitric oxide( NO ), malondialdehyde( MDA ), total superoxide dismutase( T SOD )and glutathione peroxidase( GSH - Px ) were detected after reperfusion for 2 h and 4 h.RESULTS : Compared with normal group and sham group, there were obvious changes of ST - T segment and Q wave in the electrocardiogram of I/R group.The blood level of CK - MB, cTnI, NO, MDA and GSH - Px in I/R group increased( P ＜0.05 ,P ＜0.01 ) after reperfusion for 2 h and 4 h, and the hlood level of T - SOD in I/R group after reperfusion for 2 h and 4 h also increased ( P ＜0.05 ).The heart infarct size in I/R group was the largest as compared to other groups.Among these groups, the apoptotic index of I/R group was the highest and the Bcl - 2/Bax ratio in I/R group decreased( P ＜ 0.01 ).CONCLUSION : The rat model of heart ischemia - reperfusion injury in vivo can be successfully established with the " U - shape tube" .There are obviously changes of heart infarct size. blood level of CK - MB, cTnI, NO, MDA, T - SOD and GSH - Px, myocardial apoptotic index and Bcl - 2/Bax ratio hetween I/R rats and control animals.关键词
大鼠/再灌注损伤/细胞凋亡分类
医药卫生引用本文复制引用
邓宇珺,杨敏,谭卫萍,苏健,符永恒,谭宁,曾红科,单志新,林秋雄,李晓红,董小莉,余细勇..大鼠活体心肌缺血再灌注损伤模型的建立与综合评估[J].中国病理生理杂志,2011,27(2):412-416,5.基金项目
广东省科技计划资助项目(No.2008B030301166) （No.2008B030301166）
广东省自然科学基金资助项目( No.9151008002000002) （ No.9151008002000002）
