中国病理生理杂志2011，Vol.27Issue(2)：272-277,6.DOI:10.3969/j.issn.1000-4718.2011.02-012
CIK细胞回输联合吉西他滨和顺铂治疗鼻咽癌放疗后肝肺转移瘤的效果及机制研究
Efficacy of cytokine-induced killer cell transfusion combined with chemotherapy using gemcitabine and cisplatin for nasopharyngeal carcinoma with hepatic or pulmonary metastasis
摘要
Abstract
AIM : To evaluate the recent and long - term efficacy of cytokine - induced killer( CIK ) cell and transfusion plus chemotherapy with gemcitabine and cisplatin( GP ) for patients of nasopharyngeal carcinoma( NPC ) with hepatic or pulmonary metastasis.METHODS: From Aug.2007 to Jul.2008, 30 patients of nasopharyngeal carcinoma with hepatic or pulmonary metastasis were enrolled in the study and randomly divided into 3 groups.Ten patients( CIK + GP group ) received CIK cell transfusion plus GP chemotherapy for 4 cycles.Another 10 patients( GP group )received the regimen of GP chemotherapy alone.The other 10 patients as well as 10 healthy volunteers did not receive any anti - cancer treatment.The early response, the changes of serum EBV - DNA by quantitative PCR detection and distribution of lympholeukocyte subset in the patients of the 3 groups were determined before and after treatment.The long - term effects were observed in a follow - up process of 2 vears.RESULTS : The recent complete and partial response( CR + PR ) rates were 90％, 70％ and 10％ in CIK + GP group, GP group and patient control group, respectively, and obviously statistical differences among these 3 groups were observed.The proportion of CD3 + lymphocytes in NPC patients with hepatic or pulmonary metastasis were obviously lower than that in healthy volunteer.It also descended after CIK + GP/GP therapy, but partly recovered in CIK + GP group.Furthermore, no statistical difference of CD4 + /CD8+ ratio among NPC patients with hepatic or pulmonary metastasis and volunteers was found.The ratio of CD4 + /CD8+ in CIK + GP group increased.but decreased in GP group after treatment.In the 2 - year follow - up, the overall survival rate was 60.0％ , 40.0％ and 20.0％in CIK + GP, GP and control group , respectively.The Kaplan - Meier survival curves showed statistical difference.CONCLUSION : The recent and long - term effects of CIK cell transfusion comhined with GP chemotherapy for patients of NPC with hepatic or pulmonary metastasis have been confirmed.The chemotherapy and biotherapy of CIK + GP improve the prognosis with synergistic effects, possibly owing to the changed proportion of CD3 + and ratio of CD4 + /CD8+ .关键词
鼻咽肿瘤/转移/肝肿瘤/肺肿瘤/细胞因子诱导杀伤细胞/过继性细胞免疫疗法/吉西他滨/顺铂分类
医药卫生引用本文复制引用
黎建军,古模发,刘立志,陈诗萍,张蓉,李永强..CIK细胞回输联合吉西他滨和顺铂治疗鼻咽癌放疗后肝肺转移瘤的效果及机制研究[J].中国病理生理杂志,2011,27(2):272-277,6.基金项目
广东省科技计划项目基金(社会发展)资助项目(No.2006B36002026 （社会发展）
No.2007B030700001) （）
广东省自然科学基金资助项目(No.303040801003) （No.303040801003）
