中国病理生理杂志2011，Vol.27Issue(2)：350-356,7.DOI:10.3969/j.issn.1000-4718.2011.02-026
DMT1在多巴胺能神经元变性中的作用研究
Role of divalent metal transporter 1 in degeneration of dopaminergic neuron
摘要
Abstract
AIM : To observe the expression of divalent metal transporter 1( DMTI ) in SH - SY5Y cells with lactacystin -induced injury, and to investigate the possible role of DMT1 in the degeneration of dopaminergic neuron in Parkinson disease( PD ).METHODS : An in vitro model of cell injury was established in SH - SY5Y cells induced by lactacystin.The protein expression of DMT1 was detected by immunofluorescence and Western blotting.Under the environment of high iron level, the cellular oxidative stress was observed by fluorescent probe DCFH - DA.The level of α - synuclein was determined by immunohistochemistry and Western blotting.RESULTS: The cell viability rate was reduced by lactacystin in a concentration - dependent pattern.Compared with the control, the protein level of DMT1 was obviously increased in lactacystin - treated cells.The arrangements of the changes from high to low in decreased cell viability, increased intracellular oxidative stress and increased aggregation of α - synuclein( 43 - 55 kD ) were Fe2+ treatment group ＞lactacystin treatment group ＞ control group( P ＜ 0.05 ).CONCLUSION : Lactacystin up - regulates the protein expression of DMT1.By this way, the increased function of DMT1 - mediated iron uptake may play an important role in iron or iron - catalyzed oxidative reactions to enhance α - synuclein aggregation, leading to the degeneration of neurons in PD.关键词
二价金属离子转运蛋白1/二价铁/乳胞素/SH-SY5Y细胞/α-突触核蛋白/氧化性应激/帕金森病分类
医药卫生引用本文复制引用
陈瑛,竺飞燕,王炼,包琼琼,刘云,胡乔,张雄..DMT1在多巴胺能神经元变性中的作用研究[J].中国病理生理杂志,2011,27(2):350-356,7.基金项目
国家自然科学基金资助项目(No.30670748) （No.30670748）
浙江省自然科学基金资助项目(No.Y207557) （No.Y207557）
浙江省中医药局资助项目(No.2010ZB103) （No.2010ZB103）
