中国药理学通报2011，Vol.27Issue(2)：281-284,4.
巴戟天糖链对缺氧复氧损伤内皮细胞的血管形成作用研究
Angiogenesis effects of Morinda officinalis oligosaccharides on human umbilical vein endothelial cells induced by hypoxia-reoxygenation injury
摘要
Abstract
Aim To investigate the angiogenesis effects of MOO on HUVECS induced by hypoxia-reoxygenation ( H/R )injury.Methods The models of H/R injury were made with the 3 ～ 8 generation of cultured HUVECS.Cultured HUVECS were divided into six groups: sham group; hypoxia-reoxygenation ( H/R ) group; H/R + SBW; H/R + low dose of MOO( 0.15 g· L-1 ); H/R + medium dose of MOO( 0.15 g · L-1 );H/R + high dose of MOO( 0.45 g· L-1 ).The state of HUVECS was observed with inverted microscope; cell cycle distribution was examined by flow cytometry ( FCM ) ; transwell chamber migration assays were used to calculate the number of migrated cells; matrigel tubule formation assays were employed to assess the area of tube formation.Results Compared with sham group , H/R increased the ratio of G2+ S phase ; but the MOO decreased the Ratio of G2+S phase; the MOO increased the number of cell migration; tube formation in the area of MOO groups was increased.Conclusion MOO can improve the state of cells induced by H/R,inhibit apoptosis and impact the angiogenesis of HUVEC induced by H/R.关键词
巴戟天糖链/内皮细胞/缺氧复氧损伤/流式细胞检测/细胞迁移/小管形成分类
医药卫生引用本文复制引用
王宁,周鹏,冯国清,刘心玉,张培君,吴斌,胡香杰,乔鹏..巴戟天糖链对缺氧复氧损伤内皮细胞的血管形成作用研究[J].中国药理学通报,2011,27(2):281-284,4.基金项目
河南省重点科技攻关项目(No 072102330041) （No 072102330041）
