中国药理学通报2011，Vol.27Issue(2)：162-166,5.
缺氧环境对PC12细胞损伤及HIF-1mRNA表达水平的影响
Anoxic injury and HIF-1 mRNA expression changes after hypoxia in PC12 cell
摘要
Abstract
Aim To investigate the effect of the anoxic injury and HIF-1 mRNA expression changes after hypoxia in PC12 cell.Methods A hypoxia model was established; The inverted contrast phase microscope was used to observe cell morphology; MTT assay was used to determine the effect of normal and hypoxic environment on PC12 cell proliferation; Anaerobic incubation of PC12 cell lasted for 0.2,4,8.12 , 24 and 48 h.before lactate dehydrogenase( LDH ) activity was measured; Real-time RT-PCR detected the expression of HIF-1 mRNA.Results Anaerobic cultivation after 24 h,the proliferation of PC12 cells was inhibited ( P ＜0.05 ), hypoxia after 48 h the proliferation of PC12 cells was inhibited significantly( P ＜ 0.01 ) ; PC12 cells hypoxia 2,4.8,12.24 and 48 h.the cell culture medium LDH activity increased in a time-dependant manner within the 2 -8 h range, and gradually decreases after the 8 h peak( P ＜ 0.01 ); the expression of HIF-1 mRNA level grodually increased within the 0～4 h range ,and dropped after the 4 h peak.Coriclusion Hypoxia environmental can cause PC12 cell damage, hypoxia 8 h damaged membrane most seriously, hypoxia 4 h the HIF-1 mRNA expression reached the highest level.关键词
PC12细胞/缺氧损伤/MTT/乳酸脱氢酶/HIF-1 mRNA/基因表达分类
医药卫生引用本文复制引用
姚娟,马慧萍,杨燕,樊鹏程,景临林,贾正平..缺氧环境对PC12细胞损伤及HIF-1mRNA表达水平的影响[J].中国药理学通报,2011,27(2):162-166,5.基金项目
国家科技部重大专项资助项目(No 2008ZXJ09004-52,2008ZXJ09014-010) （No 2008ZXJ09004-52,2008ZXJ09014-010）
