中国药理学通报2011，Vol.27Issue(2)：200-205,6.
二烯丙基二硫下调LIMK1抑制人结肠癌SW480细胞迁移与侵袭
Diallyl disulfide inhibits migration and invasion in human colon cancer SW480 cells by downregulating expression of LIMK1
摘要
Abstract
Aim To investigate the effects of DADS on expression of LIMK1 and the influences on migration and invasion in human colon carcinoma SW480 cells.And to elucidate the molecular mechanism of the inhibitive effect of Diallyl disulfide on migration and invasion in cancer cells.Methods The effects of DADS on expression of LIMK1 in SW480 cells was detected by Immunocytochemistry, RT-PCR and Western blot.Migration potentials were examined by Wound Healing assay.Invasion potentials were determined by transwell membrane assays.Results Compared with the negative control group,the expression of LIMK1 mRNA was inhibited significantly in SW480 cells after treated with DADS examined by RT-PCR.LIMK1 protein was overexpressed in human Colon cancer SW480 cell lines.detected by Immunocytochemistry.And its expression was remarkably downregulated after treatment with DADS.Moreover.Western bolt showed that DADS reduced expressions of LIMK1 protein in a time-dose dependent manner.Wound Healing assays showed that as the increase of concentration of DADS.the scratch healing distances were broadening.The results indicated that DADS can inhibit the capability of migration in SW480 cell lines in a dose-dependent manner.Transwell invasion assays revealed that with the increase of quantities of DADS.the numbers of invaded cells were obviously decreased.Its results exhibited that DADS can inhibit the capability of invasion in SW480 cell lines in a dosedependent manner.Conclusions After treatment with DADS , we can see significant inhibitory expression levels of LIMK1 , suppression of migration and invasion capability in SW480 cell lines.The potential molecule mechanisms may be that DADS can downregulate expression levels of LIMK1.关键词
二烯丙基二硫/结肠癌/SW480细胞/LIMK1/迁移/侵袭分类
医药卫生引用本文复制引用
史玲,苏坚,廖前进,杨晶,夏红,苏琦..二烯丙基二硫下调LIMK1抑制人结肠癌SW480细胞迁移与侵袭[J].中国药理学通报,2011,27(2):200-205,6.基金项目
国家自然科学基金资助项目(No 31000629) （No 31000629）
湖南省教育厅科学研究重点项目(No 09A077) （No 09A077）
湖南省卫生厅科研计划项目(No B2008-067,B2010-038) （No B2008-067,B2010-038）
