中国药理学通报2011，Vol.27Issue(1)：121-124,4.
铁包金总黄酮体内对S180实体瘤的抑制作用
Research on the in vivo anti-tumor effect of flavone from Berchemia lineata(L)DC
摘要
Abstract
Aim To study the anti-tumor effects of the flavone from Berchemia lineata( L )DC in vivo and discuss its possible mechanisms.Methods A total of 50 cases of S180 mice were assigned randomly into 5 groups : saline group( control ), Cisplatin group( positive control )and flavone high, middle and low group.The anti-tumor effect on internal organ of mice was observed to study the tumor inhibition rate in different groups.Influence of flavone on mouse immune system was studied by comparing the index of thymus, spleen and liver.SOD & MDA was detected in serum.The growth and patho-morphologic changes of tumor cells were observed through pathological sections.The protein expression level of p53, TNF-a and Caspase-3 were detected by immunohistochemistry.Results ①The inhibitory rate was 50.35％,35.66％ ,23.08％ in the flavone group and 83.22％ in the positive control group respectively.Mouse thymus , spleen , liver index in flavone group had no significant difference compared with that of the control group( P＞ 0.05 ).However, positive thymus spleen,liver index in control group had a significant decrease compared with that in other groups ( P＜ 0.01 ).② The flavone group of mouse SOD significantly increased and MDA reduced.③ Pa thology revealed that necrotic area of dose groups and the positive group was larger than the control group.④ Immunohistochemistry showed that p53 protein expres sion was increased in dose groups , TNF-α and Caspase 3 protein expression decreased gradually.Conclusion The flavone can scanvenge oxygen free radicals and adjust express of p53, TNF-α and Caspase-3 to sup press tumor growth.关键词
铁包金/总黄酮/S180/SOD/MDA/病理切片/免疫组化分类
医药卫生引用本文复制引用
陈小龙,滕红丽,沈玉霞,梅之南..铁包金总黄酮体内对S180实体瘤的抑制作用[J].中国药理学通报,2011,27(1):121-124,4.基金项目
广西科学基金资助课题(No 0991006) （No 0991006）
